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Oct4 antibody [7E7]

Anti-Oct4 antibody [7E7] used in Flow cytometry (FACS). GTX82755
Anti-Oct4 antibody [7E7] used in Western Blot (WB). GTX82755
Anti-Oct4 antibody [7E7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82755

Cat. No. GTX82755

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 7E7

Isotype

 IgG1

Application

 WB, ICC/IF, FACS, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all Oct4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthesized peptide derived from internal of human Oct4.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

POU class 5 homeobox 1 , OCT3 , OCT4 , OTF-3 , OTF3 , OTF4 , Oct-3 , Oct-4

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013]

Database

Research Area

DATA IMAGES

Anti-Oct4 antibody [7E7] used in Flow cytometry (FACS). GTX82755

GTX82755 FACS Image

FACS analysis of Jurkat cells using GTX82755 Oct4 antibody [7E7].
Green : Oct4
Purple : negative control

Anti-Oct4 antibody [7E7] used in Western Blot (WB). GTX82755

GTX82755 WB Image

WB analysis of NTERA-2 cell lysate using GTX82755 Oct4 antibody [7E7].

Anti-Oct4 antibody [7E7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82755

GTX82755 ICC/IF Image

ICC/IF analysis of NTERA-2 cells using GTX82755 Oct4 antibody [7E7].
Green : Oct4
Red: Actin filaments

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399