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Oct4 antibody [C-10]

Cat. No. GTX01778

Reference 5

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 C-10

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP

Reactivity

 Human, Mouse, Rat
Package
500 μl ($419)
See all Oct4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P 1:50-1:100
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent

Note :

IHC-P
Pretreatment of deparaffinized tissue with heat-induced epitope retrieval or enzymatic retrieval is recommended.
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

Seminoma

Product Note

This antibody is recommended for detection of Oct-3A (Oct-4) and Oct-3B of mouse, rat and human.

PROPERTIES

Form

Liquid

Buffer

Tris-HCl, 0.2% BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Amino acids 1-134 of OCT-4 of human origin

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

POU class 5 homeobox 1 , OCT3 , OCT4 , OTF-3 , OTF3 , OTF4 , Oct-3 , Oct-4

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013]

Database

Research Area

REFERENCE

Clone Reference

2018; Differentiation-Dependent Regulation of Human Endogenous Retrovirus K Sequences and Neighboring Genes in Germ Cell Tumor Cells
Application : WB
Reactivity : Human
2017; Transplantation of iPS-Derived Tumor Cells with a Homozygous MHC Haplotype Induces GRP94 Antibody Production in MHC-Matched Macaques
Application : IHC-P
2017; A Chemical-Genetic Approach Reveals the Distinct Roles of GSK3α and GSK3β in Regulating Embryonic Stem Cell Fate
Application : ICC/IF
Reactivity : Mouse
2015; Characterization of mesenchymal stem cells derived from the equine synovial fluid and membrane
Application : FACS
Reactivity : Mouse
2009; Neuroprotective effects of mesenchymal stem cells derived from human embryonic stem cells in transient focal cerebral ischemia in rats
Application : IHC-Fr
Reactivity : Rat
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Package List Price ($)
$ 419