APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
1:50-1:200 |
1:1000-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
LGNSO transfected HeLa , human ESC
Predict Reactivity
Rabbit, Bovine, Cat, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 20% Glycerol
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.66 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
From tissue culture supernatant
Conjugation
Unconjugated
RRID
AB_11176459
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
POU class 5 homeobox 1 , OCT3 , OCT4 , OTF-3 , OTF3 , OTF4 , Oct-3 , Oct-4
Cellular Localization
Nucleus
Background
This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX627419 IP Image
Oct4 antibody immunoprecipitates Oct4 protein in IP experiments. IP Sample: cell lysate/extract of Oct4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of Oct4 by 2 μg of Oct4 antibody (GTX627419) 12% SDS-PAGE The immunoprecipitated Oct4 protein was detected by Oct4 antibody (GTX627419) diluted at 1:1000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.
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GTX627419 IHC-P Image
Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using OCT3/4(GTX627419) antibody at 1:200 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX627419 WB Image
Oct4 antibody [GT486] detects Oct4 protein by western blot analysis. A. 30 μg human ESC whole cell lysate/extract 10% SDS-PAGE (GTX627419) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX627419 IHC-P Image
Oct4 antibody [GT486] detects Oct4 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human cervical carcinoma.Oct4 stained by Oct4 antibody [GT486] (GTX627419) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX627419 WB Image
Mouse embryonic stem cell extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] (GTX627419) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX627419 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] (GTX627419) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX627419 ICC/IF Image
Oct4 antibody detects Oct4 protein by immunofluorescent analysis. Sample: human embryonic stem cell were fixed in 4% paraformaldehyde for 15 min. Green: Oct4 protein stained by Oct4 antibody (GTX627419) diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
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GTX627419 WB Image
Sample (10 μg of whole cell lysate) A: Non-transfected HeLa lysates B: HeLa transfected recombinant Oct4 protein 10% SDS PAGE GTX627419 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX627419 FACS Image
Oct4 antibody [GT486] detects POU5F protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Oct4 antibody [GT486] dilution: 1:50
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GTX627419 ICC/IF Image
Oct4 antibody [GT486] detects Oct4 protein at nucleus by immunofluorescent analysis.Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Oct4 stained by Oct4 antibody [GT486] (GTX627419) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar= 10 μm.
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GTX627419 WB Image
Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] (GTX627419) diluted at 1:120000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX627419 IHC-P Image
Oct4 antibody [GT486] detects Oct4 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.Oct4 stained by Oct4 antibody [GT486] (GTX627419) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
REVIEW
Oct4 antibody [GT486]
Cat. No. GTX627419
Rating
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( Average 4.3 based on 3 users reviews)
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Application
Western Blot(WB)
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( Average 4.5 based on 2 users reviews)
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Flow cytometry(FACS)
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( Average 4 based on 1 users reviews)
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Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Cancers (Basel) in 2019.
PMID: 31878324
Date :
Anonymous submitted on 17-Jun-2016
Application Tested :
WB
Sample Species :
Hu
Sample :
HeLa cells
Amount used :
60μg
Blocking :
5% milk, 24°C, 1.5Hr
Primary Antibody :
1:1200, 4°C, 16Hr
Date :
Anonymous submitted on 16-May-2013
Application Tested :
FACS
Sample Species :
Hu
Sample :
hES cells
Blocking :
2% serum, 4°C, 0.5Hr
Fixation :
70% ethanol
Primary Antibody :
1:50, 4°C, 0.5Hr