Oct4 antibody [GT735]

Oct4 antibody immunoprecipitates Oct4 protein in IP experiments. IP Sample: cell lysate/extract of Oct4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of Oct4 by 2 μg of Oct4 antibody (GTX627423) 12% SDS-PAGE The immunoprecipitated Oct4 protein was detected by Oct4 antibody (GTX627423) diluted at 1:1000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.

Oct4 antibody detects Oct4 protein by immunofluorescent analysis.
Sample: human embryonic stem cell were fixed in 4% paraformaldehyde for 15 min.
Green: Oct4 protein stained by Oct4 antibody (GTX627423) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Sample (30 ug of whole cell lysate)
A: NT2D1
10% SDS PAGE
GTX627423 diluted at 1:1000

Oct4 antibody [GT735] detects POU5F protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Oct4 antibody [GT735] dilution: 1:50

Oct4 antibody [GT735] detects Oct4 protein by Western blot analysis.
A. 30 μg human ESC whole cell lysate/extract
10 % SDS-PAGE
(GTX627423) dilution: 1:1000

Sample (10 ug of whole cell lysate)
A: Non-transfected HeLa lysates
B: OCT 3/4 transfected HeLa lysates
10% SDS PAGE
GTX627423 diluted at 1:5000

Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT735] (GTX627423) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.