Oct4 antibody [GT735]

Name: Oct4 antibody [GT735]
Brand: GeneTex
SKU: GTX627423
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-Oct4 antibody [GT735] used in Immunoprecipitation (IP). GTX627423

Anti-Oct4 antibody [GT735] used in Western Blot (WB). GTX627423

Anti-Oct4 antibody [GT735] used in Flow cytometry (FACS). GTX627423

Anti-Oct4 antibody [GT735] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX627423

Cat. No. GTX627423

Host	Mouse
Clonality	Monoclonal
Clone Name	GT735
Isotype	IgG1
Application	WB, ICC/IF, FACS, IP
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all Oct4 products

Datasheet
Document

View product citations for antibody GTX627423 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
FACS	1:50-1:200
IP	1:100-1:500

Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

NT2D1 , OCT 3/4 transfected HeLa , human ESC

Predict Reactivity

Rabbit, Bovine, Cat, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888015

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

POU class 5 homeobox 1 , OCT3 , OCT4 , OTF-3 , OTF3 , OTF4 , Oct-3 , Oct-4

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]

Database

Gene ID: 5460 POU5F1
UniProt: Q01860 POU5F1

Research Area

DATA IMAGES

GTX627423 IP Image

Oct4 antibody immunoprecipitates Oct4 protein in IP experiments. IP Sample: cell lysate/extract of Oct4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of Oct4 by 2 μg of Oct4 antibody (GTX627423) 12% SDS-PAGE The immunoprecipitated Oct4 protein was detected by Oct4 antibody (GTX627423) diluted at 1:1000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.

GTX627423 WB Image

Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT735] (GTX627423) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX627423 WB Image

Sample (10 ug of whole cell lysate)
A: Non-transfected HeLa lysates
B: OCT 3/4 transfected HeLa lysates
10% SDS PAGE
GTX627423 diluted at 1:5000

GTX627423 WB Image

Oct4 antibody [GT735] detects Oct4 protein by Western blot analysis.
A. 30 μg human ESC whole cell lysate/extract
10 % SDS-PAGE
(GTX627423) dilution: 1:1000

GTX627423 FACS Image

Oct4 antibody [GT735] detects POU5F protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Oct4 antibody [GT735] dilution: 1:50

GTX627423 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
10% SDS PAGE
GTX627423 diluted at 1:1000

GTX627423 ICC/IF Image

Oct4 antibody detects Oct4 protein by immunofluorescent analysis.
Sample: human embryonic stem cell were fixed in 4% paraformaldehyde for 15 min.
Green: Oct4 protein stained by Oct4 antibody (GTX627423) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

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REVIEW