Oct4 antibody [GT735]

Name: Oct4 antibody [GT735]
Brand: GeneTex
SKU: GTX627423
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-Oct4 antibody [GT735] used in Immunoprecipitation (IP). GTX627423

Anti-Oct4 antibody [GT735] used in Western Blot (WB). GTX627423

Anti-Oct4 antibody [GT735] used in Flow cytometry (FACS). GTX627423

Anti-Oct4 antibody [GT735] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX627423

Cat. No. GTX627423

Host	Mouse
Clonality	Monoclonal
Clone Name	GT735
Isotype	IgG1
Application	WB, ICC/IF, FACS, IP
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all Oct4 products

Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
FACS	1:50-1:200
IP	1:100-1:500

Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

NT2D1 , OCT 3/4 transfected HeLa , human ESC

Predict Reactivity

Rabbit, Bovine, Cat, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888015

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

POU class 5 homeobox 1 , OCT3 , OCT4 , OTF-3 , OTF3 , OTF4 , Oct-3 , Oct-4

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]

Database

Gene ID: 5460 POU5F1
UniProt: Q01860 POU5F1

Research Area

DATA IMAGES

GTX627423 IP Image

Oct4 antibody immunoprecipitates Oct4 protein in IP experiments. IP Sample: cell lysate/extract of Oct4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of Oct4 by 2 μg of Oct4 antibody (GTX627423) 12% SDS-PAGE The immunoprecipitated Oct4 protein was detected by Oct4 antibody (GTX627423) diluted at 1:1000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.

GTX627423 WB Image

Oct4 antibody [GT735] detects Oct4 protein by Western blot analysis.
A. 30 μg human ESC whole cell lysate/extract
10 % SDS-PAGE
(GTX627423) dilution: 1:1000

GTX627423 FACS Image

Oct4 antibody [GT735] detects POU5F protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Oct4 antibody [GT735] dilution: 1:50

GTX627423 WB Image

Sample (10 ug of whole cell lysate)
A: Non-transfected HeLa lysates
B: OCT 3/4 transfected HeLa lysates
10% SDS PAGE
GTX627423 diluted at 1:5000

GTX627423 ICC/IF Image

Oct4 antibody detects Oct4 protein by immunofluorescent analysis.
Sample: human embryonic stem cell were fixed in 4% paraformaldehyde for 15 min.
Green: Oct4 protein stained by Oct4 antibody (GTX627423) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX627423 WB Image

Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT735] (GTX627423) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX627423 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
10% SDS PAGE
GTX627423 diluted at 1:1000

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