APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000-1:10000 |
1:100-1:1000 |
1:50-1:200 |
1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
NT2D1 , OCT 3/4 transfected HeLa , human ESC
Predict Reactivity
Rabbit, Bovine, Cat, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888015
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
POU class 5 homeobox 1 , OCT3 , OCT4 , OTF-3 , OTF3 , OTF4 , Oct-3 , Oct-4
Cellular Localization
Nucleus
Background
This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX627423 IP Image
Oct4 antibody immunoprecipitates Oct4 protein in IP experiments. IP Sample: cell lysate/extract of Oct4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of Oct4 by 2 μg of Oct4 antibody (GTX627423) 12% SDS-PAGE The immunoprecipitated Oct4 protein was detected by Oct4 antibody (GTX627423) diluted at 1:1000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.
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GTX627423 WB Image
Oct4 antibody [GT735] detects Oct4 protein by Western blot analysis. A. 30 μg human ESC whole cell lysate/extract 10 % SDS-PAGE (GTX627423) dilution: 1:1000
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GTX627423 FACS Image
Oct4 antibody [GT735] detects POU5F protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Oct4 antibody [GT735] dilution: 1:50
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GTX627423 WB Image
Sample (10 ug of whole cell lysate) A: Non-transfected HeLa lysates B: OCT 3/4 transfected HeLa lysates 10% SDS PAGE GTX627423 diluted at 1:5000
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GTX627423 ICC/IF Image
Oct4 antibody detects Oct4 protein by immunofluorescent analysis. Sample: human embryonic stem cell were fixed in 4% paraformaldehyde for 15 min. Green: Oct4 protein stained by Oct4 antibody (GTX627423) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
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GTX627423 WB Image
Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT735] (GTX627423) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX627423 WB Image
Sample (30 ug of whole cell lysate) A: NT2D1 10% SDS PAGE GTX627423 diluted at 1:1000
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REFERENCE
There are currently no references for Oct4 antibody [GT735] (GTX627423). Be the first to share your publications with this product.
REVIEW
Oct4 antibody [GT735]
Cat. No. GTX627423
Rating
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( Average 4 based on 1 users reviews)
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Application
Flow cytometry(FACS)
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( Average 4 based on 1 users reviews)
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Date :
Anonymous submitted on 11-Mar-2013
Application Tested :
FACS
Sample Species :
Hu
Sample :
hES cells
Blocking :
2% serum, 4°C, 0.5Hr
Fixation :
70% ethanol
Primary Antibody :
1:50, 4°C, 0.5Hr
Figure Note :
Black : Isotype control, Green : OCT4 antibody