GeneTex
United States (US)

Oct4 antibody

GTX100468_39862_CT_FACS_19011506_681.jpg
GTX100468_39862_WB_hESC_19011506_206.jpg
GTX100468_39862_IFA_hESC_19011506_448.jpg

Cat No. GTX100468

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, FACS, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
FACS 1:50-1:200
IP Assay dependent
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

human ESC

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Cat, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Pou Class 5 Homeobox 1,Oct3,Oct4,Otf-3,Otf3,Otf4,Oct-3,Oct-4,Pou5F1

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100468_39862_CT_FACS_19011506_681.jpg

GTX100468 FACS Image

Flow cytometry on human embryonic stem cells, staining with Oct4 (GTX100468)antibody at 1:100 dilution(purple) or rabbit IgG (black).

GTX100468_39862_WB_hESC_19011506_206.jpg

GTX100468 WB Image

Sample (20 ug of whole cell lysate)
A: human ESC
10% SDS PAGE
GTX100468 diluted at 1:500

GTX100468_39862_IFA_hESC_19011506_448.jpg

GTX100468 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using Oct4(GTX100468) antibody at 1:80 dilution.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169