*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
Mouse, Rat, Rabbit, Bovine, Cat, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.62mg/ml(Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Pou Class 5 Homeobox 1,Oct3,Oct4,Otf-3,Otf3,Otf4,Oct-3,Oct-4,Pou5F1
This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]
GTX100468 FACS Image
Flow cytometry on human embryonic stem cells, staining with Oct4 (GTX100468)antibody at 1:100 dilution(purple) or rabbit IgG (black).
GTX100468 WB Image
Sample (20 ug of whole cell lysate)
A: human ESC
10% SDS PAGE
GTX100468 diluted at 1:500
GTX100468 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using Oct4(GTX100468) antibody at 1:80 dilution.