GeneTex

  • Country / Location Selection

United States (US)

Oct4 antibody

KO/KD
Anti-Oct4 antibody used in Flow cytometry (FACS). GTX100468
Anti-Oct4 antibody used in Western Blot (WB). GTX100468
Anti-Oct4 antibody used in Western Blot (WB). GTX100468
Anti-Oct4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100468

Cat. No. GTX100468

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS, IP

Reactivity

 Human, Frog
Package
100 μl ($399),
25 μl ($169)
See all Oct4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
FACS 1:50-1:200
IP Assay dependent
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

Human ESC

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Cat, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037562

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

POU class 5 homeobox 1 , OCT3 , OCT4 , OTF-3 , OTF3 , OTF4 , Oct-3 , Oct-4

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Oct4 antibody used in Flow cytometry (FACS). GTX100468

GTX100468 FACS Image

Flow cytometry on human embryonic stem cells, staining with Oct4 (GTX100468)antibody at 1:100 dilution(purple) or rabbit IgG (black).

Anti-Oct4 antibody used in Western Blot (WB). GTX100468

GTX100468 WB Image

KO/KD

Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody (GTX100468) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Oct4 antibody used in Western Blot (WB). GTX100468

GTX100468 WB Image

Sample (20 ug of whole cell lysate)
A: human ESC
10% SDS PAGE
GTX100468 diluted at 1:500

Anti-Oct4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100468

GTX100468 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using Oct4(GTX100468) antibody at 1:80 dilution.

REFERENCE

Application Reference

Gupta A et al. Life (Basel) 2021; 11 (12) Activation of the Hippo Pathway in Rana sylvatica: Yapping Stops in Response to Anoxia.
Application : WB
Reactivity : Frog
Yao X et al. Dev Cell 2018; 45 (4) : 526-536.e5 Tild-CRISPR Allows for Efficient and Precise Gene Knockin in Mouse and Human Cells.
Application : ICC/IF
Reactivity : Human
Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : IP
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for Oct4 antibody (GTX100468). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169