Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody (GTX100468) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Flow cytometry on human embryonic stem cells, staining with Oct4 (GTX100468)antibody at 1:100 dilution(purple) or rabbit IgG (black).

Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using Oct4(GTX100468) antibody at 1:80 dilution.

Sample (20 ug of whole cell lysate)
A: human ESC
10% SDS PAGE
GTX100468 diluted at 1:500