GeneTex
United States (US)

Oct4 antibody

GTX100622_40660_IP_19011506_529.jpg
GTX100622_40128_IFA_19011506_466.jpg
GTX100622_40128_IFA_hESC_19011506_544.jpg
GTX100622_40128_FACS_19011506_402.jpg
GTX100622_WB_19011506_872.jpg
GTX100622_40128_WB_M_ESC_19011506_748.jpg
GTX100622_40128_WB_hESC_19011506_560.jpg
GTX100622_40660_WB_19011506_473.jpg
GTX100622_40128_IFA2_19011506_536.jpg

Cat No. GTX100622

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, FACS, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:20000
ICC/IF 1:100-1:1000
FACS 1:50-1:200
IP 1:500-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji , HeLa nucleus , Human ESC , mouse ESC

Predict Reactivity

Rabbit, Bovine, Cat, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Pou Class 5 Homeobox 1,Oct3,Oct4,Otf-3,Otf3,Otf4,Oct-3,Oct-4,Pou5F1

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100622_40660_IP_19011506_529.jpg

GTX100622 IP Image

Oct4 antibody immunoprecipitates Oct4 protein in IP experiments. IP Sample: cell lysate/extract of Oct4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Oct4 by 2 μg of Oct4 antibody (GTX100622) 12% SDS-PAGE The immunoprecipitated Oct4 protein was detected by Oct4 antibody (GTX100622) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX100622_40128_IFA_19011506_466.jpg

GTX100622 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed human embryonic stem cell, using Oct4(GTX100622) antibody at 1:200 dilution.

GTX100622_40128_IFA_hESC_19011506_544.jpg

GTX100622 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using Oct4(GTX100622) antibody at 1:400 dilution.

GTX100622_40128_FACS_19011506_402.jpg

GTX100622 FACS Image

Flow cytometry on human embryonic stem cells, staining with Oct4 (GTX100622)antibody at 1:50 dilution(green) or rabbit IgG (black).

GTX100622_WB_19011506_872.jpg

GTX100622 WB Image

Sample (20 µg)
A: HeLa nucleus
10% SDS PAGE
GTX100622 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100622_40128_WB_M_ESC_19011506_748.jpg

GTX100622 WB Image

Sample (20 µg of whole cell lysate)
A: mouse ESC
12% SDS PAGE
GTX100622 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100622_40128_WB_hESC_19011506_560.jpg

GTX100622 WB Image

Sample (20 µg of whole cell lysate)
A: Human ESC
10% SDS PAGE
GTX100622 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100622_40660_WB_19011506_473.jpg

GTX100622 WB Image

OCT4 antibody detects OCT4 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with OCT4 antibody (GTX100622) diluted at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100622_40128_IFA2_19011506_536.jpg

GTX100622 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed human embryonic stem cell, using Oct4(GTX100622) antibody at 1:100 dilution.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169