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Oct4 antibody

Anti-Oct4 antibody used in Immunoprecipitation (IP). GTX100622
Anti-Oct4 antibody used in Western Blot (WB). GTX100622
Anti-Oct4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100622
Anti-Oct4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100622
Anti-Oct4 antibody used in Flow cytometry (FACS). GTX100622
Anti-Oct4 antibody used in Western Blot (WB). GTX100622
Anti-Oct4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100622
Anti-Oct4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100622
Anti-Oct4 antibody used in Western Blot (WB). GTX100622

Cat No. GTX100622

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, FACS, IP

Reactivity

Human, Mouse
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:20000
ICC/IF 1:100-1:1000
FACS 1:50-1:200
IP 1:500-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji , HeLa nucleus , Human ESC , mouse ESC

Predict Reactivity

Rabbit, Bovine, Cat, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.32 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037563

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

POU class 5 homeobox 1 , OCT3 , OCT4 , OTF-3 , OTF3 , OTF4 , Oct-3 , Oct-4

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Oct4 antibody used in Immunoprecipitation (IP). GTX100622

GTX100622 IP Image

Oct4 antibody immunoprecipitates Oct4 protein in IP experiments. IP Sample: cell lysate/extract of Oct4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Oct4 by 2 μg of Oct4 antibody (GTX100622) 12% SDS-PAGE The immunoprecipitated Oct4 protein was detected by Oct4 antibody (GTX100622) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Oct4 antibody used in Western Blot (WB). GTX100622

GTX100622 WB Image

Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody (GTX100622) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Oct4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100622

GTX100622 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed human embryonic stem cell, using Oct4(GTX100622) antibody at 1:200 dilution.

Anti-Oct4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100622

GTX100622 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using Oct4(GTX100622) antibody at 1:400 dilution.

Anti-Oct4 antibody used in Flow cytometry (FACS). GTX100622

GTX100622 FACS Image

Flow cytometry on human embryonic stem cells, staining with Oct4 (GTX100622)antibody at 1:50 dilution(green) or rabbit IgG (black).

Anti-Oct4 antibody used in Western Blot (WB). GTX100622

GTX100622 WB Image

Sample (20 μg of whole cell lysate)
A: mouse ESC
12% SDS PAGE
GTX100622 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Oct4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100622

GTX100622 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed human embryonic stem cell, using Oct4(GTX100622) antibody at 1:100 dilution.

Anti-Oct4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100622

GTX100622 ICC/IF Image

Oct4 antibody detects Oct4 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Oct4 stained by Oct4 antibody (GTX100622) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar= 10 μm.

Anti-Oct4 antibody used in Western Blot (WB). GTX100622

GTX100622 WB Image

Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody (GTX100622) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 23642364

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169