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Ogg1 antibody

Anti-Ogg1 antibody used in IHC (Paraffin sections) (IHC-P). GTX20204
Anti-Ogg1 antibody used in Western Blot (WB). GTX20204
Anti-Ogg1 antibody used in Western Blot (WB). GTX20204
Anti-Ogg1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20204
Anti-Ogg1 antibody used in Western Blot (WB). GTX20204

Cat. No. GTX20204

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA

Reactivity

 Human, Mouse, Rat, Primate
Package
100 μl ($449)
See all Ogg1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P Assay dependent
IHC-Fr 1:25 - 1:100
FACS Assay dependent
IP 1:10 - 1:500
ELISA Assay dependent
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A peptide derived from human Ogg1 (within amino acids 1-100). [UniProt# O15527]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_384030

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

8-oxoguanine DNA glycosylase , HMMH , HOGG1 , MUTM , OGH1

Cellular Localization

nucleoplasm,Nucleus speckle

Background

This gene encodes the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage. Alternative splicing of the C-terminal region of this gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N-terminal region in common, which contains a mitochondrial targeting signal that is essential for mitochondrial localization. Many alternative splice variants for this gene have been described, but the full-length nature for every variant has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Ogg1 antibody used in IHC (Paraffin sections) (IHC-P). GTX20204

GTX20204 IHC-P Image

IHC-P analysis of human tonsillar germinal center and mantle zone using GTX20204 Ogg1 antibody.

Anti-Ogg1 antibody used in Western Blot (WB). GTX20204

GTX20204 WB Image

WB analysis of 1, 5, 10 and 20 ng human recombinant Ogg1 protein using GTX20204 Ogg1 antibody.

Anti-Ogg1 antibody used in Western Blot (WB). GTX20204

GTX20204 WB Image

WB analysis of Jurkat cell lysate using GTX20204 Ogg1 antibody.

Anti-Ogg1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20204

GTX20204 ICC/IF Image

ICC/IF analysis of A431 cells using GTX20204 Ogg1 antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1:200
Fixation : 10% formalin
Permibilization : 0.5% Triton-X100

Anti-Ogg1 antibody used in Western Blot (WB). GTX20204

GTX20204 WB Image

WB analysis of human recombinant Ogg1 protein using GTX20204 Ogg1 antibody.
Dilution : 1 μg/ml

REFERENCE

Application Reference

Sayed IM et al. Cells 2020; 9 (9) The DNA Glycosylase NEIL2 Suppresses Fusobacterium-Infection-Induced Inflammation and DNA Damage in Colonic Epithelial Cells.
Application : WB
Reactivity : Mouse
Baptiste BA et al. Free Radic Biol Med 2018; 124 : 149-162 Enhanced mitochondrial DNA repair of the common disease-associated variant, Ser326Cys, of hOGG1 through small molecule intervention.
Application : WB
Reactivity : Human
Wang Y et al. Exp Ther Med 2017; 13 (6) : 3397-3407 Regulation of the angiotensin II-p22phox-reactive oxygen species signaling pathway, apoptosis and 8-oxoguanine-DNA glycosylase 1 retrieval in hyperoxia-induced lung injury and fibrosis in rats.
Application : WB
Reactivity : Rat
Wang Y et al. Exp Ther Med 2017; 13 : 3397-3407 Regulation of the angiotensin II-p22phox-reactive oxygen species signaling pathway, apoptosis and 8-oxoguanine-DNA glycosylase 1 retrieval in hyperoxia-induced lung injury and fibrosis in rats.
Application : WB
Reactivity : Rat
Cividini F et al. J Biol Chem 2016; (Epub) O-GlcNAcylation of Ogg1 Impairs Oxidative Mitochondrial DNA Lesion Repair in Diabetic Hearts.
Application : WB
Reactivity : Mouse
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 449