GeneTex
United States (US)

Oncostatin M antibody [17001]

Cat No. GTX10842

Host Mouse
Clonality Monoclonal
Clone Name 17001
Isotype IgG2a
Application WB, ELISA, Neut
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ELISA Assay dependent
Neut Assay dependent
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Specificity/Sensitivity

The antibody neutralizes the biological activity of recombinant human OSM. Does not cross-react with recombinant human IL-6, IL-11, CNTF, LIF and recombinant mouse OSM.
PROPERTIES

Form

Liquid

Buffer

PBS, 5% trehalose

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Antigen Species

Human

Immunogen

recombinant human OSM expressed in Escherichia coli.

Purification

Purified immunoglobulin

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MGC20461

Background

Oncostatin M (OSM) is a growth-regulating cytokine that affects a number of tumor and normal cells. OSM was first identified by its ability to inhibit the growth of A375 melanoma cells and other human tumor cells, but not inhibit the growth of normal human fibroblasts. Recombinant human OSM is produced from a DNA sequence encoding the mature 196 amino acid residue of human OSM. Oncostatin M acts synergistically with TGF-beta-1 to inhibit the proliferation of A375 melanoma cells. Oncostatin M is secreted by macrophages and activated T lymphocytes. It affects a wide variety of normal and tumor cells. It induces an increase in LDL receptor expression and LDL uptake by hepatoma cells. OSM will induce cultured human endothelial cells to increase IL6 production. It activates synovial fibroblast-like cells to produce urokinase type plasminogen activator. Oncostatin M, LIF, GCSF, IL6 and ciliary neurotrophic factor (CNTF) are structurally related members of the same cytokine family sharing similarities in their primary amino acid sequences, predicted secondary structure, and receptor components.
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Database

Package List Price ($)
$ 289