Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
The antibody neutralizes the biological activity of recombinant human OSM. Does not cross-react with recombinant human IL-6, IL-11, CNTF, LIF and recombinant mouse OSM.
Form
Liquid
Buffer
PBS, 5% Trehalose
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
recombinant human OSM expressed in Escherichia coli.
Purification
Purified immunoglobulin
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
oncostatin M
Cellular Localization
Secreted
Background
Oncostatin M (OSM) is a growth-regulating cytokine that affects a number of tumor and normal cells. OSM was first identified by its ability to inhibit the growth of A375 melanoma cells and other human tumor cells, but not inhibit the growth of normal human fibroblasts. Recombinant human OSM is produced from a DNA sequence encoding the mature 196 amino acid residue of human OSM. Oncostatin M acts synergistically with TGF-beta-1 to inhibit the proliferation of A375 melanoma cells. Oncostatin M is secreted by macrophages and activated T lymphocytes. It affects a wide variety of normal and tumor cells. It induces an increase in LDL receptor expression and LDL uptake by hepatoma cells. OSM will induce cultured human endothelial cells to increase IL6 production. It activates synovial fibroblast-like cells to produce urokinase type plasminogen activator. Oncostatin M, LIF, GCSF, IL6 and ciliary neurotrophic factor (CNTF) are structurally related members of the same cytokine family sharing similarities in their primary amino acid sequences, predicted secondary structure, and receptor components.
Database
Research Area