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Oncostatin M antibody [GT1300]

Recombinant antibody
Anti-Oncostatin M antibody [GT1300] used in Western Blot (WB). GTX03212
Anti-Oncostatin M antibody [GT1300] used in Western Blot (WB). GTX03212
Anti-Oncostatin M antibody [GT1300] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03212
Anti-Oncostatin M antibody [GT1300] used in IHC (Paraffin sections) (IHC-P). GTX03212
Anti-Oncostatin M antibody [GT1300] used in IHC (Paraffin sections) (IHC-P). GTX03212
Anti-Oncostatin M antibody [GT1300] used in Western Blot (WB). GTX03212

Cat. No. GTX03212

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1300

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($439)
See all Oncostatin M products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Oncostatin M.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

oncostatin M

Cellular Localization

Secreted

Background

This gene encodes a member of the leukemia inhibitory factor/oncostatin-M (LIF/OSM) family of proteins. The encoded preproprotein is proteolytically processed to generate the mature protein. This protein is a secreted cytokine and growth regulator that inhibits the proliferation of a number of tumor cell lines. This protein also regulates the production of other cytokines, including interleukin 6, granulocyte-colony stimulating factor and granulocyte-macrophage colony stimulating factor in endothelial cells. This gene and the related gene, leukemia inhibitory factor, also present on chromosome 22, may have resulted from the duplication of a common ancestral gene. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-Oncostatin M antibody [GT1300] used in Western Blot (WB). GTX03212

GTX03212 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Oncostatin M antibody [GT1300] (GTX03212) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Oncostatin M antibody [GT1300] used in Western Blot (WB). GTX03212

GTX03212 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Oncostatin M antibody [GT1300] (GTX03212) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Oncostatin M antibody [GT1300] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03212

GTX03212 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX03212 Oncostatin M antibody [GT1300].
Blue : DAPI for nuclear staining
Dilution : 1:100

Anti-Oncostatin M antibody [GT1300] used in IHC (Paraffin sections) (IHC-P). GTX03212

GTX03212 IHC-P Image

IHC-P analysis of rat spleen tissue section using GTX03212 Oncostatin M antibody [GT1300].
Blue : DAPI for nuclear staining
Dilution : 1:100

Anti-Oncostatin M antibody [GT1300] used in IHC (Paraffin sections) (IHC-P). GTX03212

GTX03212 IHC-P Image

IHC-P analysis of mouse spleen tissue section using GTX03212 Oncostatin M antibody [GT1300].
Blue : DAPI for nuclear staining
Dilution : 1:100

Anti-Oncostatin M antibody [GT1300] used in Western Blot (WB). GTX03212

GTX03212 WB Image

WB analysis of various samples using GTX03212 Oncostatin M antibody [GT1300].
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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Package List Price ($)
$ 439