GeneTex
United States (US)

Opsin 3 antibody [N1], N-term

GTX108155_40632_20170214_IHC-Fr_19011507_498.jpg
GTX108155_40632_IHC_19011507_494.jpg
GTX108155_40632_WB_19011507_552.jpg

Cat No. GTX108155

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC-Fr
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.51 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Opsin 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Opsin 3,Ecpn,Ppp1R116,Opn3

Cellular Localization

Membrane; Multi-pass membrane protein

Background

Opsins are members of the guanine nucleotide-binding protein (G protein)-coupled receptor superfamily. In addition to the visual opsins, mammals possess several photoreceptive non-visual opsins that are expressed in extraocular tissues. This gene, opsin 3, is strongly expressed in brain and testis and weakly expressed in liver, placenta, heart, lung, skeletal muscle, kidney, and pancreas. The gene may also be expressed in the retina. The protein has the canonical features of a photoreceptive opsin protein. [provided by RefSeq]

Database

DATA IMAGES
GTX108155_40632_20170214_IHC-Fr_19011507_498.jpg

GTX108155 IHC-Fr Image

Opsin 3 antibody [N1], N-term detects Opsin 3 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Opsin 3 protein stained by Opsin 3 antibody [N1], N-term (GTX108155) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX108155_40632_IHC_19011507_494.jpg

GTX108155 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Opsin 3(GTX108155) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108155_40632_WB_19011507_552.jpg

GTX108155 WB Image

Sample (30 ug of whole cell lysate)
A: A431
10% SDS PAGE
GTX108155 diluted at 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169