GeneTex
United States (US)

Opsin 5 antibody [N2C1], Internal

GTX100173_39384_20170214_IHC-Fr_19011506_557.jpg
GTX100173_WB_19011506_332.jpg

Cat No. GTX100173

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-Fr
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

HeLaS3

Predict Reactivity

Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human neuropsin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Opsin 5,Gpr136,Grp136,Pgr12,Tmem13,Opn5

Cellular Localization

Membrane; Multi-pass membrane protein

Background

Opsins are members of the guanine nucleotide-binding protein (G protein)-coupled receptor superfamily. This opsin gene is expressed in the eye, brain, testes, and spinal cord. This gene belongs to the seven-exon subfamily of mammalian opsin genes that includes peropsin (RRH) and retinal G protein coupled receptor (RGR). Like these other seven-exon opsin genes, this gene may encode a protein with photoisomerase activity. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100173_39384_20170214_IHC-Fr_19011506_557.jpg

GTX100173 IHC-Fr Image

Opsin 5 antibody [N2C1], Internal detects Opsin 5 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Opsin 5 protein stained by Opsin 5 antibody [N2C1], Internal (GTX100173) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX100173_WB_19011506_332.jpg

GTX100173 WB Image

Sample(30 μg of whole cell lysate)
A:HeLa S3 (GTX14654)
10% SDS PAGE
GTX100173 diluted at 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169