APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HeLaS3
Predict Reactivity
Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Human Opsin 5. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
opsin 5 , GPR136 , GRP136 , PGR12 , TMEM13
Cellular Localization
Membrane; Multi-pass membrane protein
Background
Opsins are members of the guanine nucleotide-binding protein (G protein)-coupled receptor superfamily. This opsin gene is expressed in the eye, brain, testes, and spinal cord. This gene belongs to the seven-exon subfamily of mammalian opsin genes that includes peropsin (RRH) and retinal G protein coupled receptor (RGR). Like these other seven-exon opsin genes, this gene may encode a protein with photoisomerase activity. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-Opsin 5 antibody [N2C1], Internal used in IHC (Frozen sections) (IHC-Fr). GTX100173](/upload/website/prouct_img/middle/GTX100173/GTX100173_39384_20170214_IHC-Fr_19011506_557.jpg "Anti-Opsin 5 antibody [N2C1], Internal used in IHC (Frozen sections) (IHC-Fr). GTX100173")
|
GTX100173 IHC-Fr Image
Opsin 5 antibody [N2C1], Internal detects Opsin 5 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Opsin 5 protein stained by Opsin 5 antibody [N2C1], Internal (GTX100173) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
|
![Anti-Opsin 5 antibody [N2C1], Internal used in Western Blot (WB). GTX100173](/upload/website/prouct_img/middle/GTX100173/GTX100173_WB_19011506_332.jpg "Anti-Opsin 5 antibody [N2C1], Internal used in Western Blot (WB). GTX100173")
|
GTX100173 WB Image
Sample(30 μg of whole cell lysate)
A:HeLa S3 (GTX14654)
10% SDS PAGE
GTX100173 diluted at 1:1000
|
REFERENCE
There are currently no references for Opsin 5 antibody [N2C1], Internal (GTX100173). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Opsin 5 antibody [N2C1], Internal (GTX100173). Be the first to share your experience with this product.
![Anti-Opsin 5 antibody [N2C1], Internal used in IHC (Frozen sections) (IHC-Fr). GTX100173](/upload/website/prouct_img/small/GTX100173/GTX100173_39384_20170214_IHC-Fr_19011506_557.jpg "Anti-Opsin 5 antibody [N2C1], Internal used in IHC (Frozen sections) (IHC-Fr). GTX100173")
![Anti-Opsin 5 antibody [N2C1], Internal used in Western Blot (WB). GTX100173](/upload/website/prouct_img/small/GTX100173/GTX100173_WB_19011506_332.jpg "Anti-Opsin 5 antibody [N2C1], Internal used in Western Blot (WB). GTX100173")
![Anti-Opsin 5 antibody [N2C1], Internal used in IHC (Frozen sections) (IHC-Fr). GTX100173](/upload/website/prouct_img/normal/GTX100173/GTX100173_39384_20170214_IHC-Fr_19011506_557.jpg "Anti-Opsin 5 antibody [N2C1], Internal used in IHC (Frozen sections) (IHC-Fr). GTX100173")
![Anti-Opsin 5 antibody [N2C1], Internal used in Western Blot (WB). GTX100173](/upload/website/prouct_img/normal/GTX100173/GTX100173_WB_19011506_332.jpg "Anti-Opsin 5 antibody [N2C1], Internal used in Western Blot (WB). GTX100173")
