*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji
Mouse, Rat, Bovine, Pig, Rhesus Monkey(>80% identity)
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human Optineurin. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
optineurin , ALS12 , FIP2 , GLC1E , HIP7 , HYPL , NRP , TFIIIA-INTP
Cytoplasm , perinuclear region , Golgi apparatus
This gene encodes the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14.7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq]
GTX105447 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Optineurin(GTX105447) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX105447 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Optineurin(GTX105447) antibody at 1:200 dilution.
GTX105447 WB Image
Sample(30 μg whole cell lysate)
C:Hep G2 (GTX27900)
10% SDS PAGE
GTX105447 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX105447 IP Image
Optineurin antibody immunoprecipitates Optineurin protein in IP experiments.
IP samples: HepG2 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of Optineurin protein by 4 μg Optineurin antibody (GTX105447)
7.5 % SDS-PAGE
The immunoprecipitated Optineurin protein was detected by Optineurin antibody (GTX105447) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]