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Optineurin antibody

Orthogonal
Anti-Optineurin antibody used in IHC (Paraffin sections) (IHC-P). GTX105447
Anti-Optineurin antibody used in Western Blot (WB). GTX105447
Anti-Optineurin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105447
Anti-Optineurin antibody used in Immunoprecipitation (IP). GTX105447

Cat. No. GTX105447

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Optineurin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

A431 , H1299 , HepG2

Predict Reactivity

Mouse, Rat, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Optineurin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951068

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

optineurin , ALS12 , FIP2 , GLC1E , HIP7 , HYPL , NRP , TFIIIA-INTP

Cellular Localization

Cytoplasm , perinuclear region , Golgi apparatus

Background

This gene encodes the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14.7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Optineurin antibody used in IHC (Paraffin sections) (IHC-P). GTX105447

GTX105447 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Optineurin(GTX105447) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Optineurin antibody used in Western Blot (WB). GTX105447

GTX105447 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Optineurin antibody (GTX105447) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Optineurin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105447

GTX105447 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Optineurin(GTX105447) antibody at 1:200 dilution.

Anti-Optineurin antibody used in Immunoprecipitation (IP). GTX105447

GTX105447 IP Image

Optineurin antibody immunoprecipitates Optineurin protein in IP experiments.
IP samples: HepG2 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of Optineurin protein by 4 μg Optineurin antibody (GTX105447)
7.5 % SDS-PAGE
The immunoprecipitated Optineurin protein was detected by Optineurin antibody (GTX105447) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

REFERENCE

Application Reference

Prabakaran T et al. EMBO J 2018; (Epub) Attenuation of cGAS-STING signaling is mediated by a p62/SQSTM1-dependent autophagy pathway activated by TBK1.
Application : ICC/IF
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169