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Optineurin antibody

Anti-Optineurin antibody used in Western Blot (WB). GTX132575
Anti-Optineurin antibody used in Western Blot (WB). GTX132575
Anti-Optineurin antibody used in IHC (Paraffin sections) (IHC-P). GTX132575
Anti-Optineurin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132575

Cat No. GTX132575

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse eye

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.28 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Optineurin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

optineurin,ALS12,FIP2,GLC1E,HIP7,HYPL,NRP,TFIIIA-INTP

Cellular Localization

Cytoplasm , perinuclear region , Golgi apparatus

Background

This gene encodes the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14.7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Optineurin antibody used in Western Blot (WB). GTX132575

GTX132575 WB Image

Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Optineurin antibody (GTX132575) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Optineurin antibody used in Western Blot (WB). GTX132575

GTX132575 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Optineurin antibody (GTX132575) diluted at 1:2000.

Anti-Optineurin antibody used in IHC (Paraffin sections) (IHC-P). GTX132575

GTX132575 IHC-P Image

Optineurin antibody detects Optineurin protein at cytoplasm in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
Optineurin antibody (GTX132575) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Optineurin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132575

GTX132575 ICC/IF Image

Optineurin antibody detects Optineurin protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Optineurin protein stained by Optineurin antibody (GTX132575) diluted at 1:500.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169