APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , mouse eye
Predict Reactivity
Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.28 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Optineurin. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885141
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
optineurin , ALS12 , FIP2 , GLC1E , HIP7 , HYPL , NRP , TFIIIA-INTP
Cellular Localization
Cytoplasm , perinuclear region , Golgi apparatus
Background
This gene encodes the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14.7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX132575 WB Image
Wild-type (WT) and OPTN knockout (KO) U2OS cell extracts (30 μg) were separated by gradient gel% SDS-PAGE, and the membrane was blotted with Optineurin antibody (GTX132575) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.<br><I>Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.</I>
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GTX132575 ICC/IF Image
Optineurin antibody detects Optineurin protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Optineurin protein stained by Optineurin antibody (GTX132575) diluted at 1:500.
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GTX132575 WB Image
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Optineurin antibody (GTX132575) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX132575 IHC-P Image
Optineurin antibody detects Optineurin protein at cytoplasm in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
Optineurin antibody (GTX132575) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX132575 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Optineurin antibody (GTX132575) diluted at 1:2000.
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REFERENCE
REVIEW
Optineurin antibody
Cat. No. GTX132575
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Rating
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( Average 5 based on 2 users reviews)
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Application
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Western Blot(WB)
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( Average 5 based on 1 users reviews)
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Immunoprecipitation(IP)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 09-Jun-2022
Application Tested :
IP
Sample Species :
Hu
Sample :
U2OS cells
Lane Description :
SM: 10% starting material
UB: 10% unbound fraction
IP: IP antibody
Figure Note :
Antibody-bead conjugates were prepared by adding 1.0 μg of antibody to 500 μl of PBS with 0.01% triton X-100 in a microcentrifuge tube, together with 30 μl of protein A-Sepharose beads. One ml aliquots at 1.0 mg/ml of lysate were
incubated with an antibody-bead conjugate for ~2 hrs at 4ºC.
Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Date :
Anonymous submitted on 09-Jun-2022
Application Tested :
WB
Sample Species :
Hu
Sample :
U2OS WT and OPTN KO cells
Amount used :
30μg
Primary Antibody :
1:10000, 4°C, > 12Hr
Figure Note :
Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
