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Orai1 antibody

Anti-Orai1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85057
Anti-Orai1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85057
Anti-Orai1 antibody used in Western Blot (WB). GTX85057
Anti-Orai1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85057

Cat. No. GTX85057

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all Orai1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
IHC-P 10 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

Human Spleen Tissue Lysate(GTX27921) , Human Ovary Tissue Lysate(GTX29180) , Human Spleen Tissue Slide , Human Ovary Tissue Slide

Product Note

This antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

ORAI1 antibody was raised against an 18 amino acid synthetic peptide from near the amino terminus of human ORAI1.The immunogen is located within the first 50 amino acids of ORAI1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_10726516

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ORAI calcium release-activated calcium modulator 1 , CRACM1 , IMD9 , ORAT1 , TAM2 , TMEM142A

Cellular Localization

Cell membrane

Background

Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one form of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. ORAI1 often migrates at a higher than expected molecular weight in SDS-PAGE. This antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3.

Database

Research Area

DATA IMAGES

Anti-Orai1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85057

GTX85057 IHC-P Image

IHC-P analysis of human spleen tissue using GTX85057 Orai1 antibody.
Working concentration : 20 μg/ml

Anti-Orai1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85057

GTX85057 IHC-P Image

IHC-P analysis of human spleen tissue using GTX85057 Orai1 antibody.
Working concentration : 10 μg/ml

Anti-Orai1 antibody used in Western Blot (WB). GTX85057

GTX85057 WB Image

WB analysis of human ovary tissue lysate in the (A) absence or (B) presence of blocking peptide using GTX85057 Orai1 antibody.
Working concentration : 1 μg/ml

Anti-Orai1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85057

GTX85057 IHC-P Image

IHC-P analysis of human ovary tissue using GTX85057 Orai1 antibody.
Working concentration : 2.5 μg/ml

REFERENCE

Application Reference

Morin G et al. Hum Mutat 2014; Gain-of-Function mutation in STIM1 (p.R304W) is Associated with Stormorken Syndrome.
Application : IHC-P
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399