APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
10 μg/ml |
Assay dependent |
Assay dependent |
Note :
IHC-P
Heat induced antigen retrieval in pH 6.0 citrate buffer
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
9.7 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide, PLPDCCRQKTCSC, corresponding to amino acids 2-14 of human orexin A conjugated to KLH.
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
hypocretin neuropeptide precursor , HCRT , NRCLP1 , OX , PPOX , Orexin
Cellular Localization
Rough endoplasmic reticulum,Cytoplasmic vesicle,Cell junction,Synapse
Background
This gene encodes a hypothalamic neuropeptide precursor protein that gives rise to two mature neuropeptides, orexin A and orexin B, by proteolytic processing. Orexin A and orexin B, which bind to orphan G-protein coupled receptors HCRTR1 and HCRTR2, function in the regulation of sleep and arousal. This neuropeptide arrangement may also play a role in feeding behavior, metabolism, and homeostasis. [provided by RefSeq, Jan 2010]
Database
Research Area
DATA IMAGES
|
GTX85868 IHC-P Image
IHC-P analysis of human brain, hypothalamus tissue using GTX85868 Orexin A antibody. Antigen retrieval : Heat-induced antigen retrieval Dilution : 10 μg/ml
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REFERENCE
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REVIEW
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