APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
mouse brain , rat testis
Predict Reactivity
Human, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.23 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Orexin B. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886129
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
hypocretin neuropeptide precursor , NRCLP1 , OX , PPOX
Cellular Localization
Rough endoplasmic reticulum , Cytoplasmic vesicle , Cell junction , synapse
Background
This gene encodes a hypothalamic neuropeptide precursor protein that gives rise to two mature neuropeptides, orexin A and orexin B, by proteolytic processing. Orexin A and orexin B, which bind to orphan G-protein coupled receptors HCRTR1 and HCRTR2, function in the regulation of sleep and arousal. This neuropeptide arrangement may also play a role in feeding behavior, metabolism, and homeostasis. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX129928 WB Image
hypocretin (orexin) neuropeptide precursor antibody detects hypocretin (orexin) neuropeptide precursor protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with hypocretin (orexin) neuropeptide precursor antibody (GTX129928) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX129928 WB Image
hypocretin (orexin) neuropeptide precursor antibody detects hypocretin (orexin) neuropeptide precursor protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with hypocretin (orexin) neuropeptide precursor antibody (GTX129928) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for Orexin B antibody (GTX129928). Be the first to share your publications with this product.
REVIEW
Orexin B antibody
Cat. No. GTX129928
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Rating
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( Average 5 based on 1 users reviews)
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Application
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IHC (Paraffin sections)(IHC-P)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 29-Jan-2016
Application Tested :
IHC-P
Sample Species :
Rat
Sample :
brain tissue
Fixation :
paraformaldehyde
Primary Antibody :
1:1000, 4°C, 16Hr
