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Orexin Receptor 1 antibody

Anti-Orexin Receptor 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54847
Anti-Orexin Receptor 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54847
Anti-Orexin Receptor 1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54847
Anti-Orexin Receptor 1 antibody used in Western Blot (WB). GTX54847
Anti-Orexin Receptor 1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54847
Anti-Orexin Receptor 1 antibody used in Western Blot (WB). GTX54847
Anti-Orexin Receptor 1 antibody used in Western Blot (WB). GTX54847

Cat No. GTX54847

Host Rabbit
Clonality Polyclonal
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
Package
100μl ($329)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Reconstituted antibody containsA?phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Rat

Immunogen

Peptide (C)RNWKRPSEQLEAQH, corresponding to amino acid residues 256-269 of rat OX1R (Accession P56718). 3rd intracellular loop.

Purification

Affinity purified on immobilized antigen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Hypocretin Receptor 1,Hctr1,Hcrtr1

Background

neuropeptide receptor that binds orexin A; mediates orexin A modulation of the sleep-wake cycle [RGD, Feb 2006]

Database

Research Area

DATA IMAGES
Anti-Orexin Receptor 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54847

GTX54847 ICC/IF Image

ICC/IF analysis of Colo-205 cells using Orexin Receptor 1 antibody at a dilution of 1:200 (red). (B) Live view of the same field as in (A). C. Nuclei were stained with Hoechst 33342 (blue).

Anti-Orexin Receptor 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54847

GTX54847 IHC-P Image

IHC-P analysis of rat colon using Orexin Receptor 1 antibody at a dilution of 1:100. M: mucosa. sm: submucosa. ME: muscularis externa.

Anti-Orexin Receptor 1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54847

GTX54847 IHC-Fr Image

IHC-Fr analysis of rat brainstem using Orexin Receptor 1 antibody at a dilution of 1:50 (red) and Hoechst 33342 (blue).

Anti-Orexin Receptor 1 antibody used in Western Blot (WB). GTX54847

GTX54847 WB Image

WB analysis of mouse brain lysates using Orexin Receptor 1 antibody at a dilution of 1:200 in the (1) absence or (2) presence of blocking peptide.

Anti-Orexin Receptor 1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54847

GTX54847 IHC-Fr Image

IHC-Fr analysis of mouse septum using Orexin Receptor 1 antibody at a dilution of 1:50 (green), Parvalbumin antibody (red) and Hoechst 33342 (blue). (C) Merge of A and B.

Anti-Orexin Receptor 1 antibody used in Western Blot (WB). GTX54847

GTX54847 WB Image

WB analysis of Colo-205 (lanes 1 and 3) and HT-29 (lanes 2 and 4) lysates using Orexin Receptor 1 antibody at a dilution of 1:500 in the absence (lens 1 and 2) or presence (lens 3 and 4) of blocking peptide.

Anti-Orexin Receptor 1 antibody used in Western Blot (WB). GTX54847

GTX54847 WB Image

WB analysis of rat brain lysates using Orexin Receptor 1 antibody at a dilution of 1:500 in the (1) absence or (2) presence of blocking peptide.

REFERENCE
There are currently no references for Orexin Receptor 1 antibody (GTX54847).
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REVIEW
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Package List Price ($)
$ 329