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Orexin Receptor 1 antibody

Anti-Orexin Receptor 1 antibody used in Western Blot (WB). GTX54847
Anti-Orexin Receptor 1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54847
Anti-Orexin Receptor 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54847
Anti-Orexin Receptor 1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54847
Anti-Orexin Receptor 1 antibody used in Western Blot (WB). GTX54847
Anti-Orexin Receptor 1 antibody used in Western Blot (WB). GTX54847
Anti-Orexin Receptor 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54847

Cat. No. GTX54847

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
50 μl ($639)
See all Orexin Receptor 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Peptide (C)RNWKRPSEQLEAQH, corresponding to amino acid residues 256-269 (3rd  intracellular loop) of rat OX1R (Accession  : P56718).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hypocretin receptor 1 , Hctr1

Cellular Localization

Cell membrane

Background

neuropeptide receptor that binds orexin A; mediates orexin A modulation of the sleep-wake cycle [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-Orexin Receptor 1 antibody used in Western Blot (WB). GTX54847

GTX54847 WB Image

WB analysis of rat brain lysate using GTX54847 Orexin Receptor 1 antibody preincubated with or without immunogen peptide.
Dilution : 1:500

Anti-Orexin Receptor 1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54847

GTX54847 IHC-Fr Image

IHC-Fr analysis of mouse septum tissue using GTX54847 Orexin Receptor 1 antibody. Cell nuclei (blue) are visualized with Hoechst 33342.
Panel A : OX1R (green) appears in axonal processes (right-pointing triangles).
Panel B : Parvalbumin (red) appears in septal neurons.
Panel C : Merge of OX1R and parvalbumin suggests that orexinergic innervation covers the entire septal nucleus rather than restricted to individual neurons.
Dilution : 1:50

Anti-Orexin Receptor 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54847

GTX54847 IHC-P Image

IHC-P analysis of rat colon tissue using GTX54847 Orexin Receptor 1 antibody. Note that the stain (red-brown color) is highly specific for absorptive cells in the superior third of the intestinal glands. Immunolabeling was detected using DAB as the chromogen and hematoxilin as the counterstain.
Dilution : 1:100

Anti-Orexin Receptor 1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54847

GTX54847 IHC-Fr Image

IHC-Fr analysis of rat brain tissue using GTX54847 Orexin Receptor 1 antibody.
Red : Primary antibody
Blue : Hoechst 33342   is used as a counterstain
Dilution : 1:500

Anti-Orexin Receptor 1 antibody used in Western Blot (WB). GTX54847

GTX54847 WB Image

WB analysis of Colo-205 (lanes 1 and 3) and HT-29 (lanes 2 and 4) cell lysates using GTX54847 Orexin Receptor 1 antibody preincubated with or without immunogen peptide.
Dilution : 1:500

Anti-Orexin Receptor 1 antibody used in Western Blot (WB). GTX54847

GTX54847 WB Image

WB analysis of mouse brain tissue lysate using GTX54847 Orexin Receptor 1 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

Anti-Orexin Receptor 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54847

GTX54847 ICC/IF Image

ICC/IF analysis of PFA-fixed human colon cancer cells using GTX54847 Orexin Receptor 1 antibody.
Panel A : Primary antibody
Panel B : Live view of the same field as in (A)
Panel C : DNA dye Hoechst 33342
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 639