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Orexin Receptor 2 antibody

Anti-Orexin Receptor 2 antibody used in Western Blot (WB). GTX54848
Anti-Orexin Receptor 2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54848
Anti-Orexin Receptor 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54848
Anti-Orexin Receptor 2 antibody used in Western Blot (WB). GTX54848

Cat. No. GTX54848

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
50 μl ($639)
See all Orexin Receptor 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.025% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Peptide (C)DRLARGRTSTESRKS, corresponding to amino acid residues 391-405 (Intracellular, C-terminus) of rat OX2R (Accession  : P56719).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hypocretin receptor 2

Cellular Localization

Cell membrane

Background

G-protein coupled receptor that binds orexin neuropeptides; involved in regulation of feeding behavior [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-Orexin Receptor 2 antibody used in Western Blot (WB). GTX54848

GTX54848 WB Image

WB analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates using GTX54848 Orexin Receptor 2 antibody preincubated with or without immunogen peptide.
Dilution : 1:400

Anti-Orexin Receptor 2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54848

GTX54848 IHC-Fr Image

IHC-Fr analysis of rat brain tissue using GTX54848 Orexin Receptor 2 antibody. Hoechst 33342 is used as  the counterstain (blue).
Panel A :  In the cerebellum, staining is present in cell bodies of both Golgi type I (Purkinje cells – white arrows) and Golgi type II (green arrows) neurons. Staining is also present in fibers in the molecular layer (ML).
Panel B :  In the parietal cortex, staining is evident in neural cell bodies.
Panel C :  In the hippocampus, staining is present in pyramidal cells in the CA1 layer. Note that both cell bodies and prolongations are stained. 
Dilution : 1:500

Anti-Orexin Receptor 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54848

GTX54848 ICC/IF Image

ICC/IF analysis of PFA-fixed human colon cancer cells using GTX54848 Orexin Receptor 2 antibody.
Panel A : Primary antibody
Panel B : Live view of the same field as in (A)
Panel C : DNA dye Hoechst 33342
Dilution : 1:500

Anti-Orexin Receptor 2 antibody used in Western Blot (WB). GTX54848

GTX54848 WB Image

WB analysis of Colo-205 (lanes 1 and 3) and HT-29 (lanes 2 and 4) cell lysates using GTX54848 Orexin Receptor 2 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 639