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Orexin Receptor 2 antibody

Anti-Orexin Receptor 2 antibody used in Immunohistochemistry (IHC). GTX54848
Anti-Orexin Receptor 2 antibody used in Western Blot (WB). GTX54848
Anti-Orexin Receptor 2 antibody used in Western Blot (WB). GTX54848
Anti-Orexin Receptor 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54848

Cat No. GTX54848

Host Rabbit
Clonality Polyclonal
Application WB, ICC/IF, IHC
Reactivity Human, Mouse, Rat
Package
100 μl ($329)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

52 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Reconstituted antibody containsA?phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Rat

Immunogen

Peptide (C)DRLARGRTSTESRKS, corresponding to amino acid residues 391-405 of rat OX2R (Accession P56719). Intracellular, C-terminus.

Purification

Affinity purified on immobilized antigen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Hypocretin Receptor 2,Hcrtr2

Background

G-protein coupled receptor that binds orexin neuropeptides; involved in regulation of feeding behavior [RGD, Feb 2006]

Database

Research Area

DATA IMAGES
Anti-Orexin Receptor 2 antibody used in Immunohistochemistry (IHC). GTX54848

GTX54848 IHC Image

IHC analysis of rat (A) cerebellum, (B) parietal cortex and (C) hippocampus using Orexin Receptor 2 antibody at a dilution of 1:50 (red) and Hoechst 33342 (blue). GL: Golgi type neurons. ML: molecular layer.

Anti-Orexin Receptor 2 antibody used in Western Blot (WB). GTX54848

GTX54848 WB Image

WB analysis of Colo-205 (lanes 1 and 3) and HT-29 (lanes 2 and 4) lysates using Orexin Receptor 2 antibody at a dilution of 1:200 in the absence (lens 1 and 2) or presence (lens 3 and 4) of blocking peptide.

Anti-Orexin Receptor 2 antibody used in Western Blot (WB). GTX54848

GTX54848 WB Image

WB analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates using Orexin Receptor 2 antibody at a dilution of 1:400 in the absence (lens 1 and 2) or presence (lens 3 and 4) of blocking peptide.

Anti-Orexin Receptor 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54848

GTX54848 ICC/IF Image

ICC/IF analysis of Colo-205 cells using Orexin Receptor 2 antibody at a dilution of 1:500 (red). (B) Live view of the same field as in (A). C. Nuclei were stained with Hoechst 33342 (blue).

REFERENCE
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Package List Price ($)
$ 329