WB analysis of various samples using GTX48803 OspB antibody.
Lane 1 : Protein ladder
Lane 2 : MBP-OspB fusion protein
Lane 3 : MBP
Loading : 0.1 μg
Dilution : 1:1000

Western blot showing detection of 0.1 μg of recombinant OspB protein. Lane 1: Molecular weight markers. Lane 2: MBP-OspB fusion protein (arrow; expected MW = 72.7 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 μm nitrocellulose. After blocking with 1% BSA-TTBS ) overnight at 4ºC, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in blocking buffer and imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).