*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
GST-tagged Osteocalcin recombinant protein , BGLAP-transfected 293T
Sheep, Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)
1XPBS (pH7), 20% Glycerol
0.025% ProClin 300
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.69 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the Center region of human Osteocalcin. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
bone gamma-carboxyglutamate protein , BGP , OC , OCN
This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product. [provided by RefSeq, Jun 2015]
GTX132703 WB Image
Recombinant Osteocalcin were separated by 10% SDS-PAGE, and the membrane was blotted with Osteocalcin antibody (GTX132703) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX132703 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Osteocalcin antibody (GTX132703) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX132703 ELISA Image
Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant full-length Osteocalcin protein at concentrations ranging from 0.938 ng/mL to 60 ng/mL. The coated protein is detected with Osteocalcin antibody (GTX132703) at 1 μg/mL.