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Osteopontin antibody [7C5H12]

Anti-Osteopontin antibody [7C5H12] used in ELISA (ELISA). GTX60672
Anti-Osteopontin antibody [7C5H12] used in IHC (Paraffin sections) (IHC-P). GTX60672
Anti-Osteopontin antibody [7C5H12] used in Western Blot (WB). GTX60672
Anti-Osteopontin antibody [7C5H12] used in IHC (Paraffin sections) (IHC-P). GTX60672

Cat. No. GTX60672

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 7C5H12

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human
Package
100 μg ($399)
See all Osteopontin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human Osteopontin (AA: 167-314) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

RRID

AB_2887997

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

secreted phosphoprotein 1 , BNSP , BSPI , ETA-1 , OPN

Cellular Localization

Secreted

Background

The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Anti-Osteopontin antibody [7C5H12] used in ELISA (ELISA). GTX60672

GTX60672 ELISA Image

ELISA analysis of antigen using GTX60672 Osteopontin antibody [7C5H12].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-Osteopontin antibody [7C5H12] used in IHC (Paraffin sections) (IHC-P). GTX60672

GTX60672 IHC-P Image

IHC-P analysis of prostate cancer tissue using GTX60672 Osteopontin antibody [7C5H12].

Anti-Osteopontin antibody [7C5H12] used in Western Blot (WB). GTX60672

GTX60672 WB Image

WB analysis of HEK293 (1) and Osteopontin (AA: 167-314)-hIgGFc transfected HEK293 (2) cell lysate using GTX60672 Osteopontin antibody [7C5H12].

Anti-Osteopontin antibody [7C5H12] used in IHC (Paraffin sections) (IHC-P). GTX60672

GTX60672 IHC-P Image

IHC-P analysis of endometrial cancer tissue using GTX60672 Osteopontin antibody [7C5H12].

REFERENCE

Application Reference

Fang Z et al. PLoS One 2013; 8 (8) : e72738 Effect of CGRP-adenoviral vector transduction on the osteoblastic differentiation of rat adipose-derived stem cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399