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Osteopontin antibody, C-term

Anti-Osteopontin antibody, C-term used in Western Blot (WB). GTX89519
Anti-Osteopontin antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89519

Cat. No. GTX89519

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all Osteopontin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5-1.5μg/ml
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89519-PEP).

Predict Reactivity

Mouse, Rat, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-DPKSKEEDKHLK, from the C Terminus of the protein sequence according to NP_001035147.1; NP_000573.1; NP_001035149.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

secreted phosphoprotein 1 , BNSP , BSPI , ETA-1 , OPN

Cellular Localization

Secreted

Background

The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Anti-Osteopontin antibody, C-term used in Western Blot (WB). GTX89519

GTX89519 WB Image

WB analysis of Molt-4 cell lysate using GTX89519 Osteopontin antibody, C-term.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer

Anti-Osteopontin antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89519

GTX89519 IHC-P Image

IHC-P analysis of human placenta using GTX89519 Osteopontin antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399