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Osteopontin antibody

Anti-Osteopontin antibody used in IHC (Paraffin sections) (IHC-P). GTX31886
Anti-Osteopontin antibody used in Western Blot (WB). GTX31886
Anti-Osteopontin antibody used in IHC (Paraffin sections) (IHC-P). GTX31886
Anti-Osteopontin antibody used in IHC (Paraffin sections) (IHC-P). GTX31886
Anti-Osteopontin antibody used in Western Blot (WB). GTX31886

Cat. No. GTX31886

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all Osteopontin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

Human Bladder Tissue Lysate(GTX24001)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Osteopontin antibody was raised against an 18 amino acid peptide near the amino terminus of human Osteopontin.The immunogen is located within amino acids 50 - 100 of Osteopontin.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

secreted phosphoprotein 1 , BNSP , BSPI , ETA-1 , OPN

Cellular Localization

Secreted

Background

The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Anti-Osteopontin antibody used in IHC (Paraffin sections) (IHC-P). GTX31886

GTX31886 IHC-P Image

IHC-P analysis of human bladder tissue using GTX31886 Osteopontin antibody.
Working concentration : 5 μg/ml

Anti-Osteopontin antibody used in Western Blot (WB). GTX31886

GTX31886 WB Image

WB analysis of human bladder tissue lysate using GTX31886 Osteopontin antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-Osteopontin antibody used in IHC (Paraffin sections) (IHC-P). GTX31886

GTX31886 IHC-P Image

IHC-P analysis of human bladder tissue using GTX31886 Osteopontin antibody.
Working concentration : 20 μg/ml

Anti-Osteopontin antibody used in IHC (Paraffin sections) (IHC-P). GTX31886

GTX31886 IHC-P Image

The data was published in the journal Front Pharmacol in 2020.PMID: 32477111

Anti-Osteopontin antibody used in Western Blot (WB). GTX31886

GTX31886 WB Image

The data was published in the 2021 in Front Genet. PMID: 35308164

REFERENCE

Application Reference

Chen S et al. Bioact Mater 2023; 25 : 176-188 Early osteoimmunomodulation by mucin hydrogels augments the healing and revascularization of rat critical-size calvarial bone defects.
Application : IHC-P
Reactivity : Rat
Yongchang Yang et al. Front Genet 2021; 12 : 831420 Exosome-Derived LncRNA TCONS_00072128 Mediated Osteogenic Differentiation and Inflammation by Caspase 8 Regulation.
Application : WB
Reactivity : Mouse
Shaalan AAM et al. Front Pharmacol 2020; 11 : 583 Supplement With Calcium or Alendronate Suppresses Osteopenia Due to Long Term Rabeprazole Treatment in Female Mice: Influence on Bone TRAP and Osteopontin Levels.
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399