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Osteopontin antibody

Anti-Osteopontin antibody used in Western Blot (WB). GTX37582
Anti-Osteopontin antibody used in IHC (Paraffin sections) (IHC-P). GTX37582
Anti-Osteopontin antibody used in IHC (Paraffin sections) (IHC-P). GTX37582
Anti-Osteopontin antibody used in IHC (Paraffin sections) (IHC-P). GTX37582

Cat No. GTX37582

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μg ($409)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-2000
ICC/IF Assay dependent
IHC-P 1:400-800
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.01M TBS pH7.4, 0.5% BSA, 0.015% ProClin™ 300, 25% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide derived between 151-210 amino acids of human Osteopontin

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_11174562

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

secreted phosphoprotein 1 , BNSP , BSPI , ETA-1 , OPN

Cellular Localization

Secreted

Background

The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Anti-Osteopontin antibody used in Western Blot (WB). GTX37582

GTX37582 WB Image

WB analysis of human colon cancer tissue lysate using GTX37582 Osteopontin antibody.

Anti-Osteopontin antibody used in IHC (Paraffin sections) (IHC-P). GTX37582

GTX37582 IHC-P Image

IHC-P analysis of human rectal carcinoma tissue using GTX37582 Osteopontin antibody.

Anti-Osteopontin antibody used in IHC (Paraffin sections) (IHC-P). GTX37582

GTX37582 IHC-P Image

IHC-P analysis of human rectum cancer tissue using GTX37582 Osteopontin antibody.

Anti-Osteopontin antibody used in IHC (Paraffin sections) (IHC-P). GTX37582

GTX37582 IHC-P Image

IHC-P analysis of mouse thyroid gland tissue using GTX37582 Osteopontin antibody.

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Proclin.pdf