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P-Cadherin antibody

Anti-P-Cadherin antibody used in Western Blot (WB). GTX113648
Anti-P-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX113648

Cat No. GTX113648

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

91 kDa. ( Note )

Positive Control

293T , *B6 mice E15.5,E18.5 embryo tissue ,

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human P-cadherin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036405

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cadherin 3 , CDHP , HJMD , PCAD

Cellular Localization

Cell membrane

Background

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. This gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene have been associated with congential hypotrichosis with juvenile macular dystrophy. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-P-Cadherin antibody used in Western Blot (WB). GTX113648

GTX113648 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
7.5% SDS PAGE
GTX113648 diluted at 1:5000

Anti-P-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX113648

GTX113648 IHC-P Image

Immunohistochemical analysis of paraffin-embedded FaDu xenograft, using P-cadherin(GTX113648) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

P-Cadherin antibody Cat No. GTX113648

Rating ( Average 4 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 13-Mar-2014
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : B6 mice E15.5,E18.5 embryo tissue
Blocking : Thermo Scientific SuperBlock Blocking Buffers, 25°C, 0.5Hr
Fixation : 4% Paraformaldehyde
Permeabilization /Antigen retrieval : Sodium citrate pH:6.0
Primary Antibody : 1:500, 4°C, 24Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169