Boiled and unboiled HepG2 membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with P Glycoprotein antibody (GTX108354) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)