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P Glycoprotein antibody

Orthogonal
Anti-P Glycoprotein antibody used in Western Blot (WB). GTX108370
Anti-P Glycoprotein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108370
Anti-P Glycoprotein antibody used in Western Blot (WB). GTX108370

Cat. No. GTX108370

Reference 19
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all P Glycoprotein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000

Note :

WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.
Not tested in other applications.

Calculated MW

141 kDa. ( Note )

Predict Reactivity

Rabbit, Sheep, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.77 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human P Glycoprotein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036138

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATP binding cassette subfamily B member 1 , ABC20 , CD243 , CLCS , GP170 , MDR1 , P-GP , PGY1

Background

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-P Glycoprotein antibody used in Western Blot (WB). GTX108370

GTX108370 WB Image

Orthogonal

Unboiled HepG2 whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with P Glycoprotein antibody (GTX108370) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

Anti-P Glycoprotein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108370

GTX108370 ICC/IF Image

P Glycoprotein antibody detects P Glycoprotein protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: P Glycoprotein protein stained by P Glycoprotein antibody (GTX108370) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-P Glycoprotein antibody used in Western Blot (WB). GTX108370

GTX108370 WB Image

The data was published in the journal Biomed Res Int in 2014. PMID: 25140317

REFERENCE

Application Reference

Hsia Y et al. Int J Nanomedicine 2023; 18 : 4253-4274 A Dual Concentration-Tailored Cytokine-Chemo Nanosystem to Alleviate Multidrug Resistance and Redirect Balance of Cancer Proliferation and Apoptosis.
Jadhao M et al. Antioxidants (Basel) 2021; 10 (6) The Long-Term DEHP Exposure Confers Multidrug Resistance of Triple-Negative Breast Cancer Cells through ABC Transporters and Intracellular ROS.
Application : WB
Reactivity : Human
Lanzillotta C et al. Prog Neurobiol 2020; : 101892 Chronic PERK induction promotes Alzheimer-like neuropathology in Down syndrome: Insights for therapeutic intervention.
Application : WB
Reactivity : Human
Chen Q et al. J Cell Physiol 2018; (Epub) Chloride channel-3 mediates multidrug resistance of cancer by upregulating P-glycoprotein expression.
Application : WB
Reactivity : Human
Yeh YH et al. J Exp Clin Cancer Res 2018; 37 (1) : 70 inflammatory * activates hif-1α-mediated epithelial-to-mesenchymal transition via pi3k/akt/mtor pathway.
Application : WB
Reactivity : Human
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Submit a Reference

REVIEW

P Glycoprotein antibody Cat. No. GTX108370

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-P Glycoprotein antibody used in Western Blot (WB). GTX108370
Date : Anonymous submitted on 28-Sep-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : H460 cells
Amount used : 15μg
Blocking : Blocking buffer, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 18Hr
Figure Note : 6% SDS-PAGE
Submit a Review
Application Reference
Hsia Y et al. Int J Nanomedicine 2023; 18:4253-4274 A Dual Concentration-Tailored Cytokine-Chemo Nanosystem to Alleviate Multidrug Resistance and Redirect Balance of Cancer Proliferation and Apoptosis.
Jadhao M et al. Antioxidants (Basel) 2021; 10 (6) The Long-Term DEHP Exposure Confers Multidrug Resistance of Triple-Negative Breast Cancer Cells through ABC Transporters and Intracellular ROS.
Application : WB
Reactivity : Human
Lanzillotta C et al. Prog Neurobiol 2020;:101892 Chronic PERK induction promotes Alzheimer-like neuropathology in Down syndrome: Insights for therapeutic intervention.
Application : WB
Reactivity : Human
Chen Q et al. J Cell Physiol 2018; (Epub) Chloride channel-3 mediates multidrug resistance of cancer by upregulating P-glycoprotein expression.
Application : WB
Reactivity : Human
Yeh YH et al. J Exp Clin Cancer Res 2018; 37 (1):70 inflammatory * activates hif-1α-mediated epithelial-to-mesenchymal transition via pi3k/akt/mtor pathway.
Application : WB
Reactivity : Human
Chang HP et al. Int J Oncol 2018; (Epub) pterostilbene modulates the suppression of multidrug resistance protein-1 and triggers autophagic and apoptotic mechanisms in *-resistant human oral cancer car cells via akt signaling.
Application : WB
Reactivity : Human
Maruhashi R et al. Biochim Biophys Acta 2017; 1865 (3):470-479 Elevation of sensitivity to anticancer agents of human lung adenocarcinoma A549 cells by knockdown of claudin-2 expression in monolayer and spheroid culture models.
Application : WB
Reactivity : Human
Lin GL et al. PLoS One 2017; 12 (10):e0185625 modulation of the mrna-binding protein hur as a novel reversal mechanism of *-triggered multidrug resistance in colorectal cancer cells.
Application : WB
Reactivity : Human
Yeh WL et al. Stem Cell Res Ther 2017; 8 (1):177 Peri-foci adipose-derived stem cells promote chemoresistance in breast cancer.
Application : WB
Reactivity : Human
Shen YF et al. Materials 2016; 9 (5):306 Hinokitiol-Loaded Mesoporous Calcium Silicate Nanoparticles Induce Apoptotic Cell Death through Regulation of the Function of MDR1 in Lung Adenocarcinoma Cells.
Application : WB
Reactivity : Human
Zhang W et al. RSC Adv 2016; 6:48072-48082 HDACI regulates the PI3K/Akt signaling pathway to reverse MCF-7/PTX resistance by inhibiting SET.
Reactivity : Human
Yi-Wen Lo et al. Journal of Cellular and Molecular Medicine 2015; 19 (4):744-59 mitochondrial proteomics with sirna knockdown to reveal acat1 and mdh2 in the development of *-resistant uterine cancer
Application : WB
Reactivity : Human
Szu-Ting Lin et al. Cellular and Molecular Life Sciences 2015; 72 (12):2395-409 pgrmc1 contributes to *-induced chemoresistance in mes-sa uterine sarcoma.
Application : WB
Reactivity : Human
Chen DR et al. Biomed Res Int 2014; 2014:532161 mesenchymal stem cell-induced * resistance in triple negative breast cancer.
Application : WB
Reactivity : Human
Zang Y et al. Cancer Biol Ther 2014; 15 (9):1142-52 Carfilzomib and oprozomib synergize with histone deacetylase inhibitors in head and neck squamous cell carcinoma models of acquired resistance to proteasome inhibitors.
Kao YT et al. KAOHSIUNG J MED SCI 2014; involvement of p38 mitogen-activated protein kinase in acquired *-resistant human urothelial carcinoma sublines
Chang JF et al. Toxicol Sci 2014; (Epub) nuclear proteomics with xrcc3 knockdown to reveal the development of *-resistant uterine cancer.
Application : WB
Reactivity : Human
Chen S et al. Mol Biosyst 2014; 10 (2):294-303 proteomic analysis of the proteins that are associated with the resistance to * in human breast cancer cells.
Application : WB
Reactivity : Human
Lin ST et al. J Proteomics 2012; 75 (18):5822-47 proteomic analysis of proteins responsible for the development of * resistance in human uterine cancer cells.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169