GeneTex
United States (US)

P2X1 antibody

GTX16925_FACS_18121410_309.jpg
GTX16925_WB_18121410_827.jpg
GTX16925_IHC_18121410_111.jpg
GTX16925_ICCIF_18121410_483.jpg

Cat No. GTX16925

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, FACS, IHC
Reactivity Human, Mouse, Rat
APPLICATION

Calculated MW

45 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Peptide CRPIYEFHGLYEEK, corresponding to amino acid residues 270-283 of human P2X1 receptor (Accession P51575 ). Extracellular loop.

Purification

Affinity purified on immobilized antigen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Purinergic Receptor P2X 1,P2X1,P2Rx1

Background

The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel with relatively high calcium permeability. Binding to ATP mediates synaptic transmission between neurons and from neurons to smooth muscle, being responsible, for example, for sympathetic vasoconstriction in small arteries, arterioles and vas deferens. Mouse studies suggest that this receptor is essential for normal male reproductive function. It is possible that the development of selective antagonists for this receptor may provide an effective non-hormonal male contraceptive pill. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX16925_FACS_18121410_309.jpg

GTX16925 FACS Image

Anti-P2X1_Receptor_(extracellular) - Indirect flow cytometry analysis of MEG-O1 cells: ___ Unstained cells.___ Cells + Anti-P2X1 Receptor (extracellular) antibody, (10 μg antibody/1x106 cells).

GTX16925_WB_18121410_827.jpg

GTX16925 WB Image

Anti-P2X1_Receptor_(extracellular) - Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates: 1, 2. Anti-P2X1 Receptor (extracellular) antibody, (1:200). 3, 4. Anti-P2X1 Receptor (extracellular) antibody, preincubated with the control peptide antigen.

GTX16925_IHC_18121410_111.jpg

GTX16925 IHC Image

Anti-P2X1_Receptor_(extracellular) - Expression of P2X1 in mouse cerebellum Immunohistochemical staining of mouse cerebellum using Anti-P2X1 Receptor (extracellular) antibody. A. Most of P2X1 labeling (green) appears in fine processes in the molecular layer (MOL) and in Purkinje cells (arrows show examples). B. DAPI is used as the counterstain (blue). C. Merge of A and B.

GTX16925_ICCIF_18121410_483.jpg

GTX16925 ICC/IF Image

Anti-P2X1_Receptor_(extracellular) - Expression of P2X1 in human SH-SYS5 cells Immunocytochemical staining of intact living human SH-SYS5 cells. A. Extracellular staining of cells with Anti-P2X1 Receptor (extracellular) antibody, (1:50) followed by goat anti-rabbit-AlexaFluor-488 secondary antibody. B. Nuclear staining DAPI as the counterstain. C. Merged images of A and B.

Package List Price ($)
$ 329