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P2X2 antibody [C3], C-term

Anti-P2X2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108534
Anti-P2X2 antibody [C3], C-term used in Western Blot (WB). GTX108534
Anti-P2X2 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108534

Cat. No. GTX108534

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all P2X2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Rat, Guinea pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.79 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human P2X2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037583

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

purinergic receptor P2X 2 , DFNA41 , P2X2

Cellular Localization

Membrane; Multi-pass membrane protein

Background

The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel. Binding to ATP mediates synaptic transmission between neurons and from neurons to smooth muscle. Six transcript variants encoding six distinct isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-P2X2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108534

GTX108534 IHC-P Image

P2X2 antibody [C3], C-term detects P2X2 protein at on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
P2X2 antibody [C3], C-term (GTX108534) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-P2X2 antibody [C3], C-term used in Western Blot (WB). GTX108534

GTX108534 WB Image

Sample (30 ug of whole cell lysate)

A: Hela

7.5% SDS PAGE

GTX108534 diluted at 1:1000

Anti-P2X2 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108534

GTX108534 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using P2X2(GTX108534) antibody at 1:200 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169