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P2X4 antibody

Anti-P2X4 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54851
Anti-P2X4 antibody used in Western Blot (WB). GTX54851

Cat. No. GTX54851

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP

Reactivity

 Human, Mouse, Rat
Package
50 μl ($639)
See all P2X4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Predict Reactivity

Chicken, Xenopus(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Peptide (C)KKYKYVEDYEQGLSGEMNQ, corresponding to amino acid residues 370-388 (Intracellular, C-terminus) of rat P2X4 receptor (Accession  : P51577).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887893

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

purinergic receptor P2X 4

Cellular Localization

Membrane

Background

ATP-activated ionotropic receptor; involved in synaptic transmission in the central nervous system [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-P2X4 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54851

GTX54851 IHC-Fr Image

IHC-Fr analysis of rat brain tissue using GTX54851 P2X4 antibody. DAPI is used as the counterstain (blue).
Panel A : P2RX4 (green) appears in fibers surrounding cell shapes (arrows).
Panel B : Calbindin 28K (red) appears in large neurons.
Panel C : Merge of P2RX4 and Calbindin 28K suggests variable density of P2RX4 expressing fibers on red nucleus neurons.

Anti-P2X4 antibody used in Western Blot (WB). GTX54851

GTX54851 WB Image

WB analysis of rat brain membrane lysate using GTX54851 P2X4 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

REFERENCE

Application Reference

Hu QQ et al. Purinergic Signal 2023; 19 (1) : 29-41 Dorsal root ganglia P2X4 and P2X7 receptors contribute to diabetes-induced hyperalgesia and the downregulation of electroacupuncture on P2X4 and P2X7.
Application : WB
Reactivity : Rat
Tang HY et al. J Physiol Sci 2020; 70 (1) : 45 Acupuncture attenuates the development of diabetic peripheral neuralgia by regulating P2X4 expression and inflammation in rat spinal microglia.
Application : IHC-Fr
Reactivity : Rat
Kim BG et al. Respir Physiol Neurobiol 2019; 271 : 103292 Effects of nanoparticles on neuroinflammation in a mouse model of asthma.
Application : WB, IHC-P
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 639