GeneTex
United States (US)

P2X7 antibody, N-term

GTX88360_988_IHC-P_18121411_318.jpg
GTX88360_2382_WB_18121411_466.jpg
GTX88360_987_IHC-P_18121411_956.jpg

Cat No. GTX88360

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
IHC-P 3.75μg/ml

Note :

Human Brain Cortex shows membranous staining of cell bodies and processes.
Not tested in other applications.

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88360-PEP).

Predict Reactivity

Mouse, Rat, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Concentration

0.50mg/ml

Antigen Species

Human

Immunogen

Peptide with sequence YETNKVTRIQSMNY-C, from the N Terminus of the protein sequence according to NP_002553.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

P2RX7 Antibody

Background

The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants have been identified, most of which fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq, Jul 2010]
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Research Area

DATA IMAGES
GTX88360_988_IHC-P_18121411_318.jpg

GTX88360 IHC-P Image

IHC-P analysis of human cortex using GTX88360 P2X7 antibody, N-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8µg/ml

GTX88360_2382_WB_18121411_466.jpg

GTX88360 WB Image

WB analysis of human brain (frontal cortex) lysate with (B) and without (A) blocking with the immunising peptide using GTX88360 P2X7 antibody, N-term.
Dilution : 0.3µg/ml
Loading : 35µg protein in RIPA buffer

GTX88360_987_IHC-P_18121411_956.jpg

GTX88360 IHC-P Image

IHC-P analysis of human kidney using GTX88360 P2X7 antibody, N-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75µg/ml