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GeneTex
United States (US)

P2X7 antibody

GTX104288_39974_20160127_IHC-P_R_19011506_725.jpg
GTX104288_39974_IHC_19011506_919.jpg
GTX104288_39974_20160808_IHC-Fr_19011506_435.jpg

Cat No. GTX104288

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
See all P2X7 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Predict Reactivity

Bovine, Dog, Guinea pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human P2X7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Purinergic Receptor P2X 7,P2X7,P2Rx7

Cellular Localization

Membrane; Multi-pass membrane protein

Background

The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants which would encode different isoforms have been identified although some fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq]

Research Area

DATA IMAGES
GTX104288_39974_20160127_IHC-P_R_19011506_725.jpg

GTX104288 IHC-P Image

P2X7 antibody detects P2X7 protein at cell membrane and cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
P2X7 antibody (GTX104288) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX104288_39974_IHC_19011506_919.jpg

GTX104288 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using P2X7(GTX104288) antibody at 1:250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX104288_39974_20160808_IHC-Fr_19011506_435.jpg

GTX104288 IHC-Fr Image

P2X7 antibody detects P2X7 protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: P2X7 protein stained by P2X7 antibody (GTX104288) diluted at 1:250.
Red: Protein kinase C alpha staining.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169