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P2X7 antibody

Anti-P2X7 antibody used in IHC (Paraffin sections) (IHC-P). GTX104288
Anti-P2X7 antibody used in IHC (Paraffin sections) (IHC-P). GTX104288
Anti-P2X7 antibody used in IHC (Frozen sections) (IHC-Fr). GTX104288

Cat. No. GTX104288

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all P2X7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Predict Reactivity

Bovine, Dog, Guinea pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human P2X7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951086

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

purinergic receptor P2X 7 , P2X7

Cellular Localization

Cell membrane

Background

The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants have been identified, most of which fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-P2X7 antibody used in IHC (Paraffin sections) (IHC-P). GTX104288

GTX104288 IHC-P Image

P2X7 antibody detects P2X7 protein at cell membrane and cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
P2X7 antibody (GTX104288) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-P2X7 antibody used in IHC (Paraffin sections) (IHC-P). GTX104288

GTX104288 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using P2X7(GTX104288) antibody at 1:250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-P2X7 antibody used in IHC (Frozen sections) (IHC-Fr). GTX104288

GTX104288 IHC-Fr Image

P2X7 antibody detects P2X7 protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: P2X7 protein stained by P2X7 antibody (GTX104288) diluted at 1:250.
Red: Protein kinase C alpha staining.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Yan Wang et al. Mol Med Rep 2021; 24 (1) Role of P2X7R in eosinophilic and non?eosinophilic chronic rhinosinusitis with nasal polyps.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169