GeneTex

  • Country / Location Selection

United States (US)

P2X7 antibody

Anti-P2X7 antibody used in Live cell imaging (LCI). GTX16827
Anti-P2X7 antibody used in Western Blot (WB). GTX16827
Anti-P2X7 antibody used in IHC (Frozen sections) (IHC-Fr). GTX16827

Cat. No. GTX16827

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr, FACS, LCI

Reactivity

 Human, Mouse, Rat
Package
50 μl ($639)
See all P2X7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
LCI Assay dependent
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Peptide KKGWMDPQSKGIQTGRC, corresponding to amino acid residues 136-152 (Extracellular loop) of mouse P2X7 receptor (Accession number Q9Z1M0).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887546

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

purinergic receptor P2X, ligand-gated ion channel, 7 , AI467586 , P2X(7) , P2X7R

Cellular Localization

Cell membrane

Database

Research Area

DATA IMAGES

Anti-P2X7 antibody used in Live cell imaging (LCI). GTX16827

GTX16827 LCI Image

Live cell imaging analysis of intact living RBL cells using GTX16827 P2X7 antibody.
Panel A : Primary antibody
Panel B : Live view of the cell
Dilution : 1:100

Anti-P2X7 antibody used in Western Blot (WB). GTX16827

GTX16827 WB Image

WB analysis of rat brain membrane (lanes 1,5), K562 (lanes 2 and 6), WEHI-231 (lanes 3 and 7), and HL-60 (lanes 4 and 8) lysates using GTX16827 P2X7 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

Anti-P2X7 antibody used in IHC (Frozen sections) (IHC-Fr). GTX16827

GTX16827 IHC-Fr Image

IHC-Fr analysis of rat pancreas tissue using GTX16827 P2X7 antibody. Staining is present in endocrine cells of the Isle of Langerhans. Hoechst 33342 is used as the counterstain.
Dilution : 1:100

REFERENCE

Application Reference

Yan Wang et al. Mol Med Rep 2021; 24 (1) Role of P2X7R in eosinophilic and non?eosinophilic chronic rhinosinusitis with nasal polyps.
Application : WB
Reactivity : Human
Nuka E et al. PLoS One 2018; 13 (9) : e0204229 ATP/P2X7 receptor signaling as a potential anti-inflammatory target of natural polyphenols.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

There are currently no reviews for P2X7 antibody (GTX16827). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 639