GeneTex
United States (US)

P2X7 antibody

GTX16827_ICCIF_18121410_623.jpg
GTX16827_WB_18121410_960.jpg
GTX16827_IHC-Fr_18121410_682.jpg

Cat No. GTX16827

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-Fr, FACS
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

Recommended Starting Dilutions: For WB: Use at a dilution of 1:200. For IHC-Fr: Use at a dilution of 1:100. For ICC/IF: Use at a dilution of 1:100. For FACS: Use 5-10 μg antibody/1x10^6 cells. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

68 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Mouse

Immunogen

Peptide KKGWMDPQSKGIQTGRC, corresponding to amino acid residues 136-152 of mouse P2X7 Receptor (Accession number Q9Z1M0). Extracellular loop.

Purification

Affinity purified on immobilized antigen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Purinergic Receptor P2X, Ligand-Gated Ion Channel, 7,Ai467586,P2X(7),P2X7R,P2Rx7

Database

Research Area

DATA IMAGES
GTX16827_ICCIF_18121410_623.jpg

GTX16827 ICC/IF Image

Anti-P2X7_Receptor_(extracellular) - Expression of P2X7 in rat RBL cells Immunocytochemical staining of intact living rat basophilic leukemia (RBL) cells. A. Extracellular staining of cells using Anti-P2X7 Receptor (extracellular) antibody, (1:100) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells.

GTX16827_WB_18121410_960.jpg

GTX16827 WB Image

Anti-P2X7_Receptor_(extracellular) - Western blot analysis of rat brain membranes (lanes 1,5) and human cell lines; K562 (lanes 2 and 6); WEHI-231 (lanes 3 and 7) and HL-60 (lanes 4 and 8): 1-4. Anti-P2X7 Receptor (extracellular) antibody, (1:200). 5-8. Anti-P2X7 Receptor (extracellular) antibody, preincubated with the control peptide antigen.

GTX16827_IHC-Fr_18121410_682.jpg

GTX16827 IHC-Fr Image

Anti-P2X7_Receptor_(extracellular) - Expression of P2X7 in rat pancreas Immunohistochemical staining of rat pancreas frozen section using Anti-P2X7 Receptor (extracellular) antibody, (1:100), followed by mouse anti-rabbit-AlexaFluor-594 secondary antibody (red). Staining is present in endocrine cells of the Isle of Langerhans. Hoechst 33342 is used as the counterstain.

REFERENCE
Package List Price ($)
$ 329