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P2X7 antibody

Anti-P2X7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31600
Anti-P2X7 antibody used in Western Blot (WB). GTX31600
Anti-P2X7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31600

Cat. No. GTX31600

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all P2X7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
ICC/IF 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

3T3 Cell Lysate , 3T3/BALB Cell Slide

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

P2RX7 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human P2RX7.The immunogen is located within the last 50 amino acids of P2RX7.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

purinergic receptor P2X 7 , P2X7

Cellular Localization

Cell membrane

Background

The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants have been identified, most of which fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-P2X7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31600

GTX31600 ICC/IF Image

ICC/IF analysis of 3T3 cells using GTX31600 P2X7 antibody.
Working concentration : 5 μg/ml

Anti-P2X7 antibody used in Western Blot (WB). GTX31600

GTX31600 WB Image

WB analysis of 3T3 cell lysate using GTX31600 P2X7 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-P2X7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31600

GTX31600 ICC/IF Image

ICC/IF analysis of 3T3 cells using GTX31600 P2X7 antibody.
Working concentration : 20 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399