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P2Y1 antibody

Anti-P2Y1 antibody used in Flow cytometry (FACS). GTX16924
Anti-P2Y1 antibody used in Western Blot (WB). GTX16924
Anti-P2Y1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX16924
Anti-P2Y1 antibody used in Live cell imaging (LCI). GTX16924

Cat. No. GTX16924

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-Fr, FACS, LCI

Reactivity

Human, Mouse, Rat
Package
50 μl ($639)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
LCI Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide SDEYLRSYFIYSMC, corresponding to amino acid residues 207-220 (2nd  extracellular loop) of human P2RY1 (Accession  : P47900).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

purinergic receptor P2Y1 , P2Y1 , SARCC

Cellular Localization

Cell membrane

Background

The product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor functions as a receptor for extracellular ATP and ADP. In platelets binding to ADP leads to mobilization of intracellular calcium ions via activation of phospholipase C, a change in platelet shape, and probably to platelet aggregation. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-P2Y1 antibody used in Flow cytometry (FACS). GTX16924

GTX16924 FACS Image

FACS analysis of Jurkat cells using GTX16924 P2Y1 antibody.
Black : Unstained cell
Green : Cell staining with primary antibody
Antibody amount : 5 μg antibody / 0.5-1 x 10⁶  cells

Anti-P2Y1 antibody used in Western Blot (WB). GTX16924

GTX16924 WB Image

WB analysis of rat brain (lanes 1 and 4), Jurkat (lanes 2 and 5) and K-562 (lanes 3 and 6) lysates using GTX16924 P2Y1 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

Anti-P2Y1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX16924

GTX16924 IHC-Fr Image

IHC-Fr analysis of mouse hippocampus tissue using GTX16924 P2Y1 antibody.
Panel A : P2RY1 (red) appears in the pyramidal layer (arrows).
Panel B : Nuclei staining using DAPI as the counterstain (blue). 
Panel C : Merged images of panels A and B.

Anti-P2Y1 antibody used in Live cell imaging (LCI). GTX16924

GTX16924 LCI Image

Live cell imaging analysis of intact living PC12 cells using GTX16924 P2Y1 antibody.
Panel A : Primary antibody
Panel B : Merge of panel A with the live view of the cell
Dilution : 1:50

REFERENCE

There are currently no references for P2Y1 antibody (GTX16924). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 639