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P2Y12 antibody

Anti-P2Y12 antibody used in Western Blot (WB). GTX54796
Anti-P2Y12 antibody used in Western Blot (WB). GTX54796
Anti-P2Y12 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54796

Cat. No. GTX54796

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr, FACS, IP

Reactivity

 Human, Mouse, Rat
Package
50 μl ($639)
See all P2Y12 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide (C)KTTRPFKTSNPKNLLGAK,  corresponding to amino acid residues 125-142 (Intracellular, 2nd  cytoplasmic loop) of human P2RY12 (Accession  : Q9H244).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

purinergic receptor P2Y12 , ADPG-R , BDPLT8 , HORK3 , P2T(AC) , P2Y(12)R , P2Y(AC) , P2Y(ADP) , P2Y(cyc) , P2Y12 , SP1999

Cellular Localization

Cell membrane

Background

The product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor is involved in platelet aggregation, and is a potential target for the treatment of thromboembolisms and other clotting disorders. Mutations in this gene are implicated in bleeding disorder, platelet type 8 (BDPLT8). Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-P2Y12 antibody used in Western Blot (WB). GTX54796

GTX54796 WB Image

WB analysis of rat brain membrane (1, 2) and human platelets (3, 4) lysates using GTX54796 P2Y12 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

Anti-P2Y12 antibody used in Western Blot (WB). GTX54796

GTX54796 WB Image

WB analysis of human platelet lysates using GTX54796 P2Y12 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

Anti-P2Y12 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54796

GTX54796 IHC-Fr Image

IHC-Fr analysis of mouse brain tissue using GTX54796 P2Y12 antibody. The region shown in this picture is the entorhinal cortex in mouse brain. The P2Y12 positive fibers (arrows) run from the corpus callosum (triangles) into the deep layers of the entorhinal cortex. Sytox green is used as the counterstain.

REFERENCE

Application Reference

Juul-Madsen K et al. Nat Commun 2024; 15 (1) : 1224 Amyloid-β aggregates activate peripheral monocytes in mild cognitive impairment.
Jin C et al. Bioeng Transl Med 2023; 8 (2) : e10448 Spinal cord tissue engineering using human primary neural progenitor cells and astrocytes.
Siegel PM et al. Sci Rep 2021; 11 (1) : 17459 P2Y12 receptor blockers are anti-inflammatory drugs inhibiting both circulating monocytes and macrophages including THP-1 cells.
Application : IHC-Fr
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 639