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P2Y2 antibody [HL2704]

Recombinant antibody Comparison Orthogonal Overexpression
Anti-P2Y2 antibody [HL2704] used in Western Blot (WB). GTX639458
Anti-P2Y2 antibody [HL2704] used in Western Blot (WB). GTX639458
Anti-P2Y2 antibody [HL2704] used in Western Blot (WB). GTX639458
Anti-P2Y2 antibody [HL2704] used in Western Blot (WB). GTX639458
Anti-P2Y2 antibody [HL2704] used in Western Blot (WB). GTX639458
Anti-P2Y2 antibody [HL2704] used in Western Blot (WB). GTX639458

Cat. No. GTX639458

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2704

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all P2Y2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human P2Y2.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

purinergic receptor P2Y2 , HP2U , P2RU1 , P2U , P2U1 , P2UR , P2Y2 , P2Y2R

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

The product of this gene belongs to the family of P2 receptors, which is activated by extracellular nucleotides and subdivided into P2X ligand-gated ion channels and P2Y G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor, found on many cell types, is activated by ATP and UTP and is reported to be overexpressed on some cancer cell types. It is involved in many cellular functions, such as proliferation, apoptosis and inflammation. Three transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Mar 2013]

Database

Research Area

DATA IMAGES

Anti-P2Y2 antibody [HL2704] used in Western Blot (WB). GTX639458

GTX639458 WB Image

Comparison Orthogonal

Boiled and unboiled HL-60 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with P2Y2 antibody [HL2704] (GTX639458) diluted at 1:1000 and competitor's antibody (competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-P2Y2 antibody [HL2704] used in Western Blot (WB). GTX639458

GTX639458 WB Image

Comparison Orthogonal Overexpression

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with P2Y2 antibody [HL2704] (GTX639458) diluted at 1:5000 and competitor's antibody (competitor) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-P2Y2 antibody [HL2704] used in Western Blot (WB). GTX639458

GTX639458 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (GTX639458) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-P2Y2 antibody [HL2704] used in Western Blot (WB). GTX639458

GTX639458 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (GTX639458) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-P2Y2 antibody [HL2704] used in Western Blot (WB). GTX639458

GTX639458 WB Image

Orthogonal Overexpression

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (GTX639458) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-P2Y2 antibody [HL2704] used in Western Blot (WB). GTX639458

GTX639458 WB Image

Orthogonal

Boiled and unboiled HL-60 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [HL2704] (GTX639458) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations