Boiled and unboiled HL-60 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [N3C3] (GTX114609) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [N3C3] (GTX114609) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

P2Y2 antibody [N3C3] detects P2Y2 protein at cell membrane by immunofluorescent analysis.
Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: P2Y2 stained by P2Y2 antibody [N3C3] (GTX114609) diluted at 1:500.
Blue: Hoechst 33342 staining.