Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with P4HA1 antibody [C1C3] (GTX118673) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with P4HA1 antibody [C1C3] (GTX118673) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

P4HA1 antibody [C1C3] detects P4HA1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
P4HA1 stained by P4HA1 antibody [C1C3] (GTX118673) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min