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P4HA1 antibody, Internal

Anti-P4HA1 antibody, Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89145
Anti-P4HA1 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89145
Anti-P4HA1 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89145

Cat. No. GTX89145

Reference 2

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μg ($399)
See all P4HA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1μg/ml
ICC/IF Assay dependent
IHC-P 5μg/ml
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89145-PEP).

Product Note

This antibody is expected to recognise both reported isoforms (NP_000908.2 and NP_001017962.1).

Predict Reactivity

Mouse, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-DFARKDEPDAFKE, from the internal region of the protein sequence according to NP_000908.2; NP_001017962.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_10723472

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

prolyl 4-hydroxylase subunit alpha 1 , P4HA

Background

This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-P4HA1 antibody, Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89145

GTX89145 ICC/IF Image

ICC/IF analysis of paraformaldehyde fixed A431 cells using GTX89145 P4HA1 antibody, Internal.
Green : Primary antibody
Blue : DAPI
Negative control: Unimmunized goat IgG
Dilution : 10μg/ml

Green : Primary antibody
Blue : DAPI
Negative control: Unimmunized goat IgG
Dilution : 10μg/ml

Anti-P4HA1 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89145

GTX89145 IHC-P Image

IHC-P analysis of human skin using GTX89145 P4HA1 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml

Anti-P4HA1 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89145

GTX89145 IHC-P Image

IHC-P analysis of human spleen using GTX89145 P4HA1 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml

REFERENCE

Application Reference

Ning Y et al. J Cancer 2021; 12 (22) : 6685-6694 Overexpression of P4HA1 associates with poor prognosis and promotes cell proliferation and metastasis of lung adenocarcinoma.
Application : IHC-P
Reactivity : Human
Ekman M et al. Lab Invest 2014; HIF-mediated metabolic switching in bladder outlet obstruction mitigates the relaxing effect of mitochondrial inhibition.
Application : WB
Reactivity : Rat
Submit a Reference

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399