APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
EL4 Cell Lysate(GTX27183)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
PA1 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human PA1.The immunogen is located within the last 50 amino acids of PA1.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
PAXIP1 associated glutamate rich protein 1 , C16orf53 , GAS , PA1
Cellular Localization
Nucleus
Background
Steroid receptor co-activators (SRCs) were initially described as nuclear receptor transcription co-activators, but they have recently been determined to co-regulate transcription initiated by other transcription factors. GAS is a recently identified glutamate-rich protein that interacts with SRC1, but not GRIP1 or AIB1, the other two members of the SRC family. GAS can also interact with the alpha subunit of the estrogen receptor (ERa), but not other receptors such as the retinoic acid receptor a, suggesting the interaction between GAS and ERa is relatively specific. Depletion of GAS by RNA interference in MCF7 cells led to a decrease in the mRNA and protein levels of ER target genes such as pS2, c-Myc and cyclin D1, indicating the role of GAS in the regulation of ER target genes. GAS has also been found to associate with an SET1-like methyltransferase complex specific for H3K4 methylation, suggesting that GAS has multiple roles in transcriptional regulation.
Database
Research Area
DATA IMAGES
|
GTX85239 WB Image
WB analysis of EL4 cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX85239 PA1 antibody.
Working concentration : 1μg/ml
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REFERENCE
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REVIEW
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