GeneTex
  • Country / Location Selection

United States (US)

PABP antibody

Anti-PABP antibody used in IHC (Paraffin sections) (IHC-P). GTX113954
Anti-PABP antibody used in Western Blot (WB). GTX113954
Anti-PABP antibody used in Western Blot (WB). GTX113954
Anti-PABP antibody used in IHC (Paraffin sections) (IHC-P). GTX113954
Anti-PABP antibody used in IHC (Paraffin sections) (IHC-P). GTX113954
Anti-PABP antibody used in IHC (Paraffin sections) (IHC-P). GTX113954
Anti-PABP antibody used in Western Blot (WB). GTX113954
Anti-PABP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113954
Anti-PABP antibody used in IHC (Paraffin sections) (IHC-P). GTX113954

Cat. No. GTX113954

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

Predict Reactivity

Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.79 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PABP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168130

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

poly(A) binding protein cytoplasmic 1 , PAB1 , PABP , PABP1 , PABPC2 , PABPL1

Cellular Localization

Cytoplasm , Nucleus

Background

The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation. In humans, the PABPs comprise a small nuclear isoform and a conserved gene family that displays at least 3 functional proteins: PABP1 (PABPC1), inducible PABP (iPABP, or PABPC4; MIM 603407), and PABP3 (PABPC3; MIM 604680). In addition, there are at least 4 pseudogenes, PABPCP1 to PABPCP4.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-PABP antibody used in IHC (Paraffin sections) (IHC-P). GTX113954

GTX113954 IHC-P Image

PABP antibody detects PABP protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
PABP antibody (GTX113954) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PABP antibody used in Western Blot (WB). GTX113954

GTX113954 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PABP antibody (GTX113954) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PABP antibody used in Western Blot (WB). GTX113954

GTX113954 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PABP antibody (GTX113954) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PABP antibody used in IHC (Paraffin sections) (IHC-P). GTX113954

GTX113954 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using PABP(GTX113954) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PABP antibody used in IHC (Paraffin sections) (IHC-P). GTX113954

GTX113954 IHC-P Image

PABP antibody detects PABP protein at cytosol on mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
PABP antibody (GTX113954) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PABP antibody used in IHC (Paraffin sections) (IHC-P). GTX113954

GTX113954 IHC-P Image

PABP antibody detects PABP protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
PABP antibody (GTX113954) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PABP antibody used in Western Blot (WB). GTX113954

GTX113954 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PABP antibody (GTX113954) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PABP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113954

GTX113954 ICC/IF Image

PABP antibody detects PABP protein at cytoplasm by immunofluorescent analysis.
Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PABP protein stained by PABP antibody (GTX113954) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-PABP antibody used in IHC (Paraffin sections) (IHC-P). GTX113954

GTX113954 IHC-P Image

PABP antibody detects PABP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
PABP stained by PABP antibody (GTX113954) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for PABP antibody (GTX113954). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for PABP antibody (GTX113954). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169