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PABPC3 antibody

Anti-PABPC3 antibody used in Western Blot (WB). GTX114611
Anti-PABPC3 antibody used in IHC (Paraffin sections) (IHC-P). GTX114611

Cat No. GTX114611

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

Jurkat , Raji , K562

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human PABPC3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11177168

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

poly(A) binding protein cytoplasmic 3 , PABP3 , PABPL3 , tPABP

Cellular Localization

Cytoplasm

Background

Messenger RNA stability and translation initiation are extensively under the control of poly(A)-binding proteins (PABP). See PABPC1 (MIM 604679) for background information.[supplied by OMIM]

Database

DATA IMAGES

Anti-PABPC3 antibody used in Western Blot (WB). GTX114611

GTX114611 WB Image

Sample (30 ug of whole cell lysate)

A: Jurkat

B: Raji

C: K562

7.5% SDS PAGE

GTX114611 diluted at 1:1000

Anti-PABPC3 antibody used in IHC (Paraffin sections) (IHC-P). GTX114611

GTX114611 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using PABPC3(GTX114611) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169