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PABPC5 antibody [N1C2]

Anti-PABPC5 antibody [N1C2] used in Western Blot (WB). GTX119883
Anti-PABPC5 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX119883
Anti-PABPC5 antibody [N1C2] used in Western Blot (WB). GTX119883
Anti-PABPC5 antibody [N1C2] used in Western Blot (WB). GTX119883
Anti-PABPC5 antibody [N1C2] used in Western Blot (WB). GTX119883

Cat. No. GTX119883

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PABPC5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

U87-MG , A431 , HeLa , HepG2 , mouse brain , rat brain

Predict Reactivity

Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PABPC5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10720169

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

poly(A) binding protein cytoplasmic 5 , PABP5

Cellular Localization

Cytoplasm

Background

This gene encodes a protein that binds to the polyA tail found at the 3' end of most eukaryotic mRNAs. It is thought to play a role in the regulation of mRNA metabolic processes in the cytoplasm. This gene is located in a gene-poor region within the X-specific 13d-sY43 subinterval of the chromosome Xq21.3/Yp11.2 homology block. It is located close to translocation breakpoints associated with premature ovarian failure, and is therefore a potential candidate gene for this disorder. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PABPC5 antibody [N1C2] used in Western Blot (WB). GTX119883

GTX119883 WB Image

PABP5 antibody detects PABP5 protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 10 % SDS-PAGE, and the membrane was blotted with PABP5 antibody (GTX119883) diluted by 1:1000.

Anti-PABPC5 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX119883

GTX119883 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using PABP5(GTX119883) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PABPC5 antibody [N1C2] used in Western Blot (WB). GTX119883

GTX119883 WB Image

PABP5 antibody detects PABP5 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PABP5 antibody (GTX119883) diluted by 1:1000.

Anti-PABPC5 antibody [N1C2] used in Western Blot (WB). GTX119883

GTX119883 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
10% SDS PAGE
GTX119883 diluted at 1:1000

Anti-PABPC5 antibody [N1C2] used in Western Blot (WB). GTX119883

GTX119883 WB Image

PABP5 antibody detects PABP5 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10 % SDS-PAGE, and the membrane was blotted with PABP5 antibody (GTX119883) diluted by 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169