GeneTex
United States (US)

PACAP antibody [N1C3]

GTX106794_WB_19011506_204.jpg
GTX106794_39959_20160126_IHC-Fr_M_19011506_373.jpg
GTX106794_39959_20180907_WB_M_spleen_19011506_226.jpg
GTX106794_39959_IHC_19011506_481.jpg

Cat No. GTX106794

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC-Fr
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

Molt-4 , Raji

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.02mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PACAP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ32987 antibody, MGC29506 antibody, PACAP antibody, pERp1 antibody, MZB1 antibody, plasma cell-induced resident endoplasmic reticulum protein antibody, plasma cell-induced resident ER protein antibody, caspase-2 binding protein antibody, plasma cell-induced ER protein 1 antibody, proapoptotic caspase adaptor protein antibody, HSPC190 antibody, proapoptotic caspase adapter protein antibody

Cellular Localization

Secreted (Potential) , Cytoplasm

Background

Associates with immunoglobulin M (IgM) heavy and light chains and promotes IgM assembly and secretion. May exert its effect by acting as a molecular chaperone or as an oxidoreductase as it displays a low level of oxidoreductase activity (By similarity). Isoform 2 may be involved in regulation of apoptosis.

Database

Research Area

DATA IMAGES
GTX106794_WB_19011506_204.jpg

GTX106794 WB Image

Sample(30 ug whole cell lysate)
A:MOLT4 (GTX27912)
B:Raji (GTX27908)
15% SDS PAGE
GTX106794 diluted at 1:1000

GTX106794_39959_20160126_IHC-Fr_M_19011506_373.jpg

GTX106794 IHC-Fr Image

PACAP antibody [N1C3] detects PACAP protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample:Frozen section of mouse brain.
PACAP antibody [N1C3] (GTX106794) diluted at 1:500.

GTX106794_39959_20180907_WB_M_spleen_19011506_226.jpg

GTX106794 WB Image

Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with PACAP antibody [N1C3] (GTX106794) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX106794_39959_IHC_19011506_481.jpg

GTX106794 IHC-P Image

PACAP antibody [N1C3] detects PACAP protein at cytosol on human normal colon mucosa with lymphocyte by immunohistochemical analysis.
Sample: Paraffin-embedded normal colon mucosa with lymphocyte.
PACAP antibody [N1C3] (GTX106794) dilution: 1:500.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169