GeneTex
United States (US)

PACE4 antibody, Internal

GTX88546_917_IHC-P_18121411_676.jpg
GTX88546_918_IHC-P_18121411_137.jpg

Cat No. GTX88546

Host Goat
Clonality Polyclonal
Isotype IgG
Application IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-P 3.75μg/ml
Not tested in other applications.

Calculated MW

106 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88546-PEP).

Specificity/Sensitivity

This antibody is expected to recognize reported isoforms a, b, g, h (NP_002561.1; NP_612192.1; NP_612193.1; NP_612194.1 resp.).

Predict Reactivity

Mouse, Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence PDCEPGTYFDSE, from the internal region of the protein sequence according to NP_002561.1; NP_612192.1; NP_612195.1; NP_612197.1; NP_612196.1; NP_612198.2; NP_612193.1; NP_612194.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Proprotein Convertase Subtilisin/Kexin Type 6,Pace4,Spc4,Pcsk6

Cellular Localization

Not secreted, remains probably in zymogen form in endoplasmic reticulum. Not secreted, remains probably in zymogen form in endoplasmic reticulum. Retained intracellularly probably through a hydrophobic cluster in their C-terminus. Retained intracellularly

Background

This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an initial autocatalytic processing event in the ER to generate a heterodimer which exits the ER and sorts to the trans-Golgi network where a second autocatalytic event takes place and the catalytic activity is acquired. The encoded protease is constitutively secreted into the extracellular matrix and expressed in many tissues, including neuroendocrine, liver, gut, and brain. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. Some of its substrates include transforming growth factor beta related proteins, proalbumin, and von Willebrand factor. This gene is thought to play a role in tumor progression and left-right patterning. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Feb 2014]

Database

DATA IMAGES
GTX88546_917_IHC-P_18121411_676.jpg

GTX88546 IHC-P Image

IHC-P analysis of human skeletal muscle using GTX88546 PACE4 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75µg/ml

GTX88546_918_IHC-P_18121411_137.jpg

GTX88546 IHC-P Image

IHC-P analysis of human testis using GTX88546 PACE4 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75µg/ml

Package List Price ($)
$ 259