GeneTex
United States (US)

PACSIN1 antibody

GTX108567_39911_IHC_19011507_771.jpg
GTX108567_39911_20170511_WB_M_R_19011507_857.jpg
GTX108567_39911_20170719_IFA_R_19011507_799.jpg
GTX108567_WB_19011507_284.jpg

Cat No. GTX108567

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

Molt-4 , mouse brain , rat brain

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7mg/ml

Antigen Species

Human

Immunogen

Full length human PACSIN1 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

KIAA1379 antibody, SDPI antibody, PACSIN1 antibody, protein kinase C and casein kinase substrate in neurons protein 1 antibody, syndapin I antibody, protein kinase C and casein kinase substrate in neurons 1 antibody

Cellular Localization

Cytoplasm (By similarity)

Database

Research Area

DATA IMAGES
GTX108567_39911_IHC_19011507_771.jpg

GTX108567 IHC-P Image

PACSIN1 antibody detects PACSIN1 protein at cytoplasm on MCF7 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded MCF7 xenograft.
PACSIN1 antibody (GTX108567) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108567_39911_20170511_WB_M_R_19011507_857.jpg

GTX108567 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PACSIN1 antibody (GTX108567) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108567_39911_20170719_IFA_R_19011507_799.jpg

GTX108567 ICC/IF Image

PACSIN1 antibody detects PACSIN1 protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PACSIN1 protein stained by PACSIN1 antibody (GTX108567) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX108567_WB_19011507_284.jpg

GTX108567 WB Image

Sample(30 μg of whole cell lysate)
A:MOLT4(GTX27912)
7.5% SDS PAGE
GTX108567 diluted at 1:500

SDS
Glycerol.pdf
Thimerosal.pdf