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PAD4 antibody, N-term

Anti-PAD4 antibody, N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89631
Anti-PAD4 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX89631
Anti-PAD4 antibody, N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89631

Cat. No. GTX89631

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, IHC-P, ELISA

Reactivity

 Human
Package
100 μg ($399)
See all PAD4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 10μg/ml
IHC-P 4-6μg/ml
ELISA 1:64000
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89631-PEP).

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence AQGTLIRVTPEQPC, from the N Terminus of the protein sequence according to NP_036519.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

peptidyl arginine deiminase 4 , PAD , PAD4 , PADI5 , PDI4 , PDI5

Cellular Localization

Cytoplasm , Nucleus

Background

This gene is a member of a gene family which encodes enzymes responsible for the conversion of arginine residues to citrulline residues. This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PAD4 antibody, N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89631

GTX89631 ICC/IF Image

ICC/IF analysis of PFA fixed U2OS cells using GTX89631 PAD4 antibody, N-term.
Green : Primary antibody
Blue : DAPI
VE-control : Unimmunized goat IgG (10μg/ml)
Permeabilization : 0.15% Triton
Dilution : 10μg/ml

Anti-PAD4 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX89631

GTX89631 IHC-P Image

IHC-P analysis of human spleen tissue using GTX89631 PAD4 antibody, N-term.
Antigen retrieval : Heat mediated citrate buffer (pH 6)
Left : No primary antibody
Right : Primary antibody, 4μg/ml

Anti-PAD4 antibody, N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89631

GTX89631 ICC/IF Image

ICC/IF analysis of PFA fixed HeLa cells using GTX89631 PAD4 antibody, N-term.
Green : Primary antibody
Blue : DAPI
VE-control : Unimmunized goat IgG (10μg/ml)
Permeabilization : 0.15% Triton
Dilution : 10μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399