Immunofluorescence analysis of paraformaldehyde-fixed A431, using PAD4(GTX113946) antibody at 1:200 dilution.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PAD4 antibody [N1N3] (GTX113946) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using PAD4(GTX113946) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min