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PAD4 antibody

Overexpression
Anti-PAD4 antibody used in Western Blot (WB). GTX113945
Anti-PAD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113945
Anti-PAD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113945
Anti-PAD4 antibody used in Western Blot (WB). GTX113945

Cat. No. GTX113945

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr, IHC-Wm

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PAD4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PAD4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037599

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

peptidyl arginine deiminase 4 , PAD , PAD4 , PADI5 , PDI4 , PDI5

Cellular Localization

Cytoplasm , Nucleus , Cytoplasmic granule

Background

This gene is a member of a gene family which encodes enzymes responsible for the conversion of arginine residues to citrulline residues. This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PAD4 antibody used in Western Blot (WB). GTX113945

GTX113945 WB Image

Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PAD4 antibody (GTX113945) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PAD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113945

GTX113945 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using PAD4(GTX113945) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PAD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX113945

GTX113945 IHC-P Image

PAD4 antibody detects PAD4 protein at cytoplasm on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
PAD4 antibody (GTX113945) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PAD4 antibody used in Western Blot (WB). GTX113945

GTX113945 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PAD4 antibody (GTX113945) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Kong X et al. J Exp Clin Cancer Res 2023; 42 (1) : 236 Fusobacterium nucleatum-triggered neutrophil extracellular traps facilitate colorectal carcinoma progression.
Indeglia A et al. Cancer Discov 2023; 13 (7) : 1696-1719 An African-Specific Variant of TP53 Reveals PADI4 as a Regulator of p53-Mediated Tumor Suppression.
Surolia R et al. JCI Insight 2021; 6 (10) NETosis in the pathogenesis of acute lung injury following cutaneous chemical burns.
Application : WB
Reactivity : Human
Martins VF et al. PLoS One 2021; 16 (1) : e0245594 Surgical site peptidylarginine deaminase 4 (PAD4), a biomarker of NETosis, correlates with insulin resistance in total joint arthroplasty patients: A preliminary report.
Application : WB
Reactivity : Human
Maronek M et al. Cells 2021; 10 (1) Extracellular DNA Correlates with Intestinal Inflammation in Chemically Induced Colitis in Mice.
Application : IHC-Wm
Reactivity : Mouse
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169